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Plasmid#220143Purposedoxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repressionDepositorInsertdCas9-Zim3-MeCP2
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTre-3GAvailable sinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHDM-Hgpm2
Plasmid#164441PurposeEncodes codon-optimized HIV gagpol for pseudovirus productionDepositorInsertCodon Optimized HIV-1 GagPol
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAS9PBKS
Plasmid#68371PurposeCbAkt promoter driven Cas9-2A-GFP (from px458)DepositorInsertCas9
UseTags2A-EGFPExpressionMammalianMutationPromoterCbAktAvailable sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pInducer10-mir-RUP-PheS
Plasmid#44011PurposeLentiviral shRNA vector (pGIPz based) for inducible knockdownDepositorInsertPheS Gly294
UseLentiviralTagsRFPExpressionMammalianMutationPromoterTRE2Available sinceJuly 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_Empty.EFS.tRFP657
Plasmid#212961PurposeLentiviral plasmid for the cloning of mature gRNAs of the RfxCas13d (CasRx) CRISPR system. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_backbone
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER11 (miR-RUG)
Plasmid#44363PurposeInducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interestDepositorInsertPheS Gly294
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Luc2-IRES-ZsGreen-W
Plasmid#164432PurposeLentiviral vector backbone encoding firefly luciferase and ZsGreenDepositorInsertsLuciferase
ZsGreen
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
PCBP1_Full-length_pGEX
Plasmid#135108PurposeRecombinant expression of PCBP1 with dual-affinity tagDepositorInsertPCBP1 (PCBP1 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPT234
Plasmid#127855PurposeRepressilator 2.0, without degradation tags, integrated triple reporterDepositorInsertsbla
mSCFP3
mKate2
mVenus
TetR
LacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationFirst 11aa derived from mCherry as fusion proteinPromoterpAmpR, pL LacO1, pL tetO1, and pRAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE792
Plasmid#167300PurposeVector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.DepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-NLS-AcrIIA4
Plasmid#113038PurposeAAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)DepositorInsertSV40 NLS-AcrIIA4
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-BirA-P2A-mCherry
Plasmid#74056Purposeretroviral expression plasmid for selfcleaving E.coli biotin ligase BirA-P2A-mCherry fusion proteinDepositorInsertBirA biotin ligase
UseRetroviralTagsP2A-mCherryExpressionMammalianMutationPromoterMSCV-LTRsAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_GFP-targeting sgRNA.EFS.tRFP657
Plasmid#212963PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_GFP targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO5.U6.DR130(CasRX)_Non-targeting sgRNA.EFS.tRFP657
Plasmid#212962PurposeLentiviral plasmid for the measurement of RfxCas13d (CasRx) nuclease activity. As a marker, the plasmid also encodes the tRFP657 fluorescence protein.DepositorInsertDR1_CasRx_non-targeting spacer
UseCRISPR and LentiviralTagsTagRFP657ExpressionMammalianMutationPromoterU6Available sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-PIK3C2A
Plasmid#161988PurposeMammalian expression vector containing PIK3C2A tagged with GFPDepositorInsertPIK3C2A (PIK3C2A Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-3
Plasmid#72345PurposeMammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP8
Plasmid#42311DepositorInsertVAMP 8 (VAMP8 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6**)-mCherry
Plasmid#168049PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
UseTagsExpressionBacterialMutationPromoterJ23101** and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLM494
Plasmid#100539PurposeCEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.DepositorInsertsGGPP synthase
phytoene desaturase
phytoene-beta carotene synthase
HMG CoA reductase
UseCre/Lox and Synthetic BiologyTagsExpressionYeastMutationPromoterPGK1, TDH3, TIP1, and ZEO1Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHLmMBP-11
Plasmid#72349PurposeMammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tagDepositorTypeEmpty backboneUseTags6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…ExpressionMammalianMutationPromoterCMV enhancer + chicken beta-actin promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only