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pMXs-IRES-blast GFP-SPNS1

Plasmid

#198571

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag GFP in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag-GFP fusion (SPNS1 Human)

Use

Retroviral

Tags

3xFlag-GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXs-3xFlag-SPNS1

Plasmid

#198568

Purpose

SPNS1 codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1 with n-terminal 3xFlag (SPNS1 Human)

Use

Retroviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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Hygro-P2A-HA-FKBP-WAPL

Plasmid

#217666

Purpose

Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

WapI (WAPL Human)

Use

Tags

dTag (FKBP36V protein)

Expression

Bacterial and Mammalian

Mutation

Promoter

Lac

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A ΔWIR

Plasmid

#215507

Purpose

Expresses GFP tagged human ATG2A with the mutation in WIPI interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A mLIR

Plasmid

#215506

Purpose

Expresses GFP tagged human ATG2A with the mutation in LC3 interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204342

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-i3-IRESWT-Gbetagamma ONE-GO

Plasmid

#204343

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-i3/Gbetagamma ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-SV40-Galpha-13 ONE-GO

Plasmid

#204332

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

SV40 Galpha-13 ONE-GO (GNA13 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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LARP1_323-509

Plasmid

#209800

Purpose

Bacterial expression of human LARP1 La-module

Depositor

Kalle Gehring

Article

Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

Insert

LARP1 La-module (LARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 323-509 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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LARP1_323-410

Plasmid

#209801

Purpose

Bacterial expression of human LARP1 LaM domain

Depositor

Kalle Gehring

Article

Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

Insert

LARP1 LaM (LARP1 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

aa 323-410 only

Promoter

T7

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1_B1ChR2_TS_mScarlet_ER

Plasmid

#195191

Purpose

Highly K+ selective channelrhodopsin

Depositor

Johannes Vierock, Peter Hegemann

Article

Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

Insert

Bilabrum sp. K+ selective channelrhodopsin 2

Use

Tags

ER-export sequence, Kir2.1 trafficking motif, and…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAG8H-GB1-EWS_171-264

Plasmid

#180466

Purpose

Expresses the construct His8-GB1-EWS_171-264 with a TEV cleavage site between GB1 and EWS_171-264

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Use

Tags

GB1 and His8

Expression

Bacterial

Mutation

deleted 1-170, 265-656

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAG8H-GFP-EWS_1-120

Plasmid

#180464

Purpose

Expresses the construct His8-GFP-EWS_1-120 with a TEV cleavage site between GFP and EWS_1-120

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Use

Tags

GFP and His8

Expression

Bacterial

Mutation

deleted 121f-656

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pAG8H-sumo-EWS_91-199

Plasmid

#180465

Purpose

Expresses the construct His8-sumo-EWS_91-199

Depositor

David Libich

Article

Johnson et al Biomol NMR Assign. 2022 Apr;16(1):67-73.

Insert

EWSR1 (EWSR1 Human)

Use

Tags

His8 and SUMO

Expression

Bacterial

Mutation

deleted 1-90, 200-656

Promoter

T7

Available since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMP71-mGFP-llo190-mc38minor

Plasmid

#174604

Purpose

expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 3 neoantigens from the MC38 tumor in genes Aatf, irgq and cpne1

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

palmitoyl-GFP C-term fusion LLO190 and minigenes of 3 neoantigens from MC38 tumor in genes Aatf irgq cpne1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-Noxa-4E-pEGFP-C1

Plasmid

#166745

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

Noxa-4E (PMAIP1 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

4 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCu-His6-Atg13[571–700] K683E, H685E, K686E-Ss(424)

Plasmid

#166855

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment mutated in K683E, H685E, K686E with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K683E, H685E, K686E

Promoter

pCu

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_CBX1_Chromo-shadow

Plasmid

#109965

Purpose

Protein expression and purification of CBX1_Chromo-shadow

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CBX1_Chromo-shadow (CBX1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK6_IRS

Plasmid

#109886

Purpose

Protein expression and purification of DOK6_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK6_IRS (DOK6 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_LASP1_LIM

Plasmid

#109780

Purpose

Protein expression and purification of LASP1_LIM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LASP1_LIM (LASP1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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