-
Plasmid#178885PurposeExpression of GST-His8-HRV3CDepositorInserthuman rhinovirus 3C protease
UseTagsGST, His8ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-174-566
Plasmid#104197PurposeExpresses human AR-174-566 in pGEX-3x bacterial expression vector as fusion protein with GSTDepositorInserthuman androgen receptor aa 174-566 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Synapsin-cyto-iATPSnFR1.0
Plasmid#102556Purposeexpresses cytoplasmic ATP sensor in neuronsDepositorInsertiATPSnFR1.0
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEW100cre_del_tetO
Plasmid#24019DepositorTypeEmpty backboneUseTrypanosome transient expressionTagsCre RecombinaseExpressionMutationPromoterAvailable sinceMay 11, 2010AvailabilityAcademic Institutions and Nonprofits only -
-
myr-FLAG-PIK3CA-pcw107
Plasmid#64605Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertPIK3CA (PIK3CA Bovine)
UseLentiviralTagsFLAG and myristoylatedExpressionMutationPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
acta1-mRuby2-dGBP
Plasmid#163855PurposeZebrafish muscle specific expression of mRuby2-dVHH[GFP]DepositorInsertdVHH[GFP]
UseIn vitro transcription of rnaTagsRuby2ExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM10 with rat FL Annexin A11
Plasmid#164496PurposeFor bacterial expression of rat His-tagged FL Annexin A11DepositorInsertrat FL Annexin A11 (Anxa11 Rat)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pblic-neo TRX1
Plasmid#110919PurposeTo overexpress Thioredoxin-1DepositorInsertTRX1 (TXN Human)
UseRetroviralTagsExpressionMutationPromoterunknownAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBS hTSP-3
Plasmid#12995DepositorInsertTSP-3 (THBS3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_RHBG
Plasmid#131883PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertRHBG (RHBG Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSynapsin_NLS_GFP-P2A_SNAP_GluA2
Plasmid#170455PurposeExpresses SNAP tagged GluA2 in neuronal cellsDepositorInsertGluA2 (Gria2 Mouse)
UseLentiviralTagsSNAPExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRU295
Plasmid#167694PurposeFinal destination vector for single Gateway LR reactionDepositorInsertpEC1.2::Cas9i
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUbC-FLAG-24xSuntagV4-oxEBFP-AID-baUTR1-24xMS2V5-Wpre
Plasmid#84561PurposepSINAPS; single molecule translation reporter containing a FLAG tag, Suntag, oxBFP, AID, beta-actin 3'UTR, and MS2V5 stem-loopsDepositorInsertFLAG tag, Suntag, oxBFP, Auxin-inducible degron, beta-actin 3'UTR, MS2V5 stem-loops
UseLentiviralTagsFlag, Suntag, oxBFP, AID (auxin-inducible degron)ExpressionMutationPromoterUbCAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV2A
Plasmid#224440PurposeRep/Cap plasmid for the production of MyoAAV 2A, a muscle-tropic AAV capsid in mice.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVTagsExpressionMutationRGDQTTL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-GalNAcT2-moxGFP2
Plasmid#218966PurposeExpresses full length GalNAcT2-moxGFP2 mammalian cellsDepositorInsertGalNAcT2-moxGFP2 (GALNT2 Human)
UseTagsmoxGFP2ExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225246PurposeMammalian expression of iPASnFR-L in the nucleusDepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-NLS-iPASnFR-H-mScarlet3-3xFLAG
Plasmid#225245PurposeMammalian expression of iPASnFR-H in the nucleusDepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-iPASnFR-L-mScarlet3-3xFLAG-Rab5
Plasmid#225244PurposeMammalian expression of iPASnFR-L fused to Rab5DepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-iPASnFR-H-mScarlet3-3xFLAG-Rab5
Plasmid#225243PurposeMammalian expression of iPASnFR-H fused to Rab5DepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-cox8-iPASnFR-L-mScarlet3-3xFLAG
Plasmid#225240PurposeMammalian expression of iPASnFR-L in mitochondriaDepositorInsertiPASnFR-L
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-iPASnFR-L
Plasmid#225236PurposeAAV expression of iPASnFR-L from hSyn promoterDepositorInsertiPASnFR-L
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-iPASnFR-L
Plasmid#225235PurposeAAV expression of iPASnFR-L from GFAP promoterDepositorInsertiPASnFR-L
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKY925F
Plasmid#216547PurposeThis retroviral plasmid expresses a mutated cDNA (dominant negative: Y925F ) of human PTK2DepositorInsertPTK2 (Y925F) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK dominant negative mutation Y925FPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLVX-puro-mGSDME D270A
Plasmid#223518PurposeExpresses mGSDME D270A in mammalian cellsDepositorInsertGSDME (Gsdme Mouse)
UseLentiviralTagsExpressionMammalianMutationD270APromoterAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-YTHDC2
Plasmid#222558PurposePiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2DepositorInsertYTHDC2 (YTHDC2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV GG hUbV-EGFP
Plasmid#216161PurposeContains a Golden-Gate cloning cassette to express up to four gRNA and EGFPDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSbi-pur-EGFP-KSR1-CA3 (N-KSR)
Plasmid#217769PurposeFluorescent reporter for glucosyl-ceramide (putative, mammalian expression)DepositorInsertKinase suppressor of ras 1 CA3 domain (KSR1 Human)
UseSleeping beauty transposase-compatible genome ins…TagsEGFPExpressionMammalianMutationCA3 domain aa 317-400PromoterEF-1alpha promoterAvailable sinceJune 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
PE-SUMO CRYAB
Plasmid#217670PurposeFor expression of SUMO tagged human alpha B crystallinDepositorInsertCRYAB (CRYAB Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1-MBP-TEV-NAP1 (WT)
Plasmid#208871PurposeFor expression in E. coli of NAP1DepositorInsertNAP1 (NAP1L1 Human)
UseTagsMBP-TEVExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSIIbleo-iRFP670-P2A-EGFP
Plasmid#198060PurposeThe lentiviral vector for iRFP670 and EGFPDepositorInsertiRFP670
UseLentiviralTagsP2A-EGFPExpressionMammalianMutationPromoterCAGAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag2B-E2F3
Plasmid#202522PurposeExpressing human E2F3 protein with FLAG-tagDepositorInsertE2F3 (E2F3 Human)
UseTagsFLAG-tagExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCas9-sgVRK1 #4-Blast
Plasmid#199647PurposeExpresses Cas9 and sgRNA guide targeting VRK1DepositorInsertN/A (VRK1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiCas9-sgCh2-2-Blast
Plasmid#199645PurposeExpresses Cas9 and sgRNA control guide targeting intergenic regionDepositorInsertN/A
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsMago_I
Plasmid#146579PurposeMammalian Expression of HsMagoDepositorInsertHsMago (MAGOH Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsMago_I
Plasmid#146582PurposeMammalian Expression of HsMagoDepositorInsertHsMago (MAGOH Human)
UseTagsExpressionMammalianMutationone non silent mutation Y123C compared to the seq…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-CD40LG-COMP5AP-AviTag-9xHis
Plasmid#157200PurposeMammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.DepositorInsertCD40LG (CD40LG Human)
UseTagsCOMP5AP-AviTag-9xHisExpressionMammalianMutationPromoterCMV/SP6Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6
Plasmid#192789PurposeaGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labelingDepositorInsertantiGFP nanobody minimizer
UseTagsH6 and ybbRExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Opto-GPR4
Plasmid#106001PurposeExpression of a chimeric G-protein coupled receptor (Rhodopsin-GPR4; Opto-GPR4; A3)DepositorInsertOpto-GPR4 (GPR4 Bovine, Human)
UseTagsRhodopsin-1D4 and VSV-GExpressionMammalianMutationChimeric receptor proteinPromoterCMVAvailable sinceJan. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
NL4-3 mCherry Luciferase
Plasmid#44965PurposeHIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.DepositorInsertmCherry, Luciferase, IRES
UseLentiviralTagsLuciferase, IRES and mCherry with D212GExpressionMutationS34C mutation in NefPromoterAvailable sinceOct. 30, 2015AvailabilityAcademic Institutions and Nonprofits only