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Plasmid#101773PurposeExpresses BAF in human cells (with EGFP produced from the same transcript as expression control)DepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFP-P2AExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FLCN (WT) FLAG GFP
Plasmid#164978PurposeLentiviral vector for FLCN expression in mammalian cellsDepositorInsertFLCN (FLCN Human)
UseLentiviralTags1xFLAG-GFPExpressionMammalianMutationPromoterUBCAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRES:mTREK-1(G137I)
Plasmid#133274PurposeMammalian expression vector. It will generate the mouse TREK-1 channel full lenght G137I mutant fused to a N-terminal HA tagDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
UseTagsHAExpressionMammalianMutationG137IPromoterCMVd2Available sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTP434
Plasmid#104167PurposeBacterial expression plasmid of anti-GFP nanobody with 3x Cysteines for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
UseTags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation3x Cysteines located at bps 493-495, 520-522, and…PromoterAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XMS2 Lentiviral Vector
Plasmid#174198PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X MS2 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviralTags24X MS2 hairpins, Blasticidin, and T2AExpressionMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gene Trap - 24XPP7 Lentiviral Vector
Plasmid#174199PurposeLentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.DepositorInsertiRFP
UseLentiviral; VectorTags24X PP7 hairpins, Blasticidin, and T2AExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
WP_010880027.1
Plasmid#194729PurposeLumazine synthase of the hyperthermophile Aquifex aeolicus bacteriumDepositorInsertnanoparticle-proteinA and His tag
UseTagsHis tagExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pre-pro-alphaf(I)-E2-Crimson
Plasmid#117660PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpre-pro-alphaf(I)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDTFendoNA2
Plasmid#174731PurposeExpresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.DepositorInsertDT386F
UseTagsExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
H14-MBP-SUMO-Cys-Linker-BAF_G47E
Plasmid#101779PurposeExpresses His-MBP-tagged mutant BAF (G47E) in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationG47E mutationPromoterT5Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES
Plasmid#182485PurposeYeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS-NES
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free PcPTS
Plasmid#182486PurposeYeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS-PTS
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked Free ClLIS1 (M)
Plasmid#182487PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-ClLIS1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)DepositorInsertFPPS(M)-LS
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL10Available sinceNov. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 2497
Plasmid#110942PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseLentiviralTagsExpressionMutationPromoterH1/TOAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-P2A_BAF_L58R
Plasmid#101775PurposeExpresses mutant BAF (L58R) in human cells (with EGFP produced from the same transcript as expression control)DepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFP-P2AExpressionMammalianMutationL58R mutationPromoterEF1aAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-pOst1-pro-alphaf(MUT1)-E2-Crimson
Plasmid#117662PurposeTest intracellular localisation of E2-Crimson and study the secretion efficiencyDepositorInsertpOst1-pro-af(MUT1)-E2-Crimson
UseTagsExpressionYeastMutationThis plasmid encodes the fluorescent protein E2-C…PromoterpAOX1Available sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 1714
Plasmid#110941PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseRNAiTagsExpressionMutationPromoterH1/TOAvailable sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 2956
Plasmid#110943PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterH1/TOAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FLCN (R164A) FLAG GFP
Plasmid#164980PurposeLentiviral vector for FLCN expression in mammalian cellsDepositorInsertFLCN (FLCN Human)
UseLentiviralTags1xFLAG-GFPExpressionMammalianMutationR164APromoterUBCAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMMACE DEST CMV SpCas9
Plasmid#206268PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.DepositorInsertSpCas9
UseCRISPR; Recombinant baculovirus productionTagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only