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Plasmid#197865PurposeDonor plasmid used for building MED6-mEmerald knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseMouse TargetingTagsmEmeraldExpressionMutationPromoterNAAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-RCaMP1h
Plasmid#191467PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) RCaMP1h to mTurquoise2 fluorescent proteinDepositorInsertRCaMP1h
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA
Plasmid#194725Purposebased on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFBDepositorArticleUseCRISPRTagsExpressionMammalianMutationD908APromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-EGFP
Plasmid#191458PurposeCompare the intensity of EGFP to mScarlet-I fluorescent proteinDepositorInsertEGFP
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mScarletI-antiFRET-P2A-mTurquoise2_T203Y
Plasmid#191459PurposeCompare the intensity of mTurquoise2_T203Y to mScarlet-I fluorescent proteinDepositorInsertmTurquoise2_T203Y
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-mCherry
Plasmid#191466PurposeCompare the intensity of mCherry to mTurquoise2 fluorescent proteinDepositorInsertmCherry
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRuby3 only VLP
Plasmid#182429PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-mRuby3
deltaVP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D5:Luc
Plasmid#193007PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D5)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D5PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D4:Luc
Plasmid#193006PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D4)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D4PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D6:Luc
Plasmid#193008PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D6)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D6PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D1:Luc
Plasmid#193003PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D1)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D1PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D3:Luc
Plasmid#193005PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D3)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D3PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
Zic4-3505-D2:Luc
Plasmid#193004PurposePlasmid to test the Hydra Zic4 promoter activity (partial deletion D2)DepositorInsertHydra Zic4 promoter
UseTagsExpressionMammalianMutationpartial deletion of the Hydra Zic4 promoter-D2PromoterAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI
Plasmid#191473PurposeNegative control for ratiometric comparison between green fluorescent proteins and sensorsDepositorInsertnone
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRESS-mTq2-antiFRET-P2A2-linker_noSacI
Plasmid#191472PurposeNegative control for ratiometric comparison between red fluorescent proteins and sensorsDepositorInsertnone
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xnls-G-Ca-FLITS
Plasmid#191463PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTags3xNLSExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MON +T111H
Plasmid#187432PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged threonine at 117 to histidinePromoterPH and p10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
TEG +H122D
Plasmid#187434PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 128 to aspartic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
FER +H111E
Plasmid#187429PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged histidine at 117 to glutamic acidPromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
KEE +E111H
Plasmid#187431PurposeExpresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cellsDepositorInsertsATP1A1
ATP1B1
UseTagsExpressionInsectMutationchanged glutamic acid at 117 to histidinePromoterPH and p10Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only