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Plasmid#44158DepositorInsertMekk2 (Map3k2 Mouse)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pY130 (pcDNA3.1-U6-huBsCpf1)
Plasmid#92306PurposeExpression of humanized BsCpf1 and crRNADepositorInserthuBsCpf1
UseTagsNLS and NLS-3xHAExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA_RGG-RFP-RGG
Plasmid#124934PurposeExpression of RGG-RFP-RGGDepositorInsertRGG-RFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_HaloTag7_SKL
Plasmid#175532PurposePeroxysomal expression of HaloTag7 in mammalian cellsDepositorInsertHaloTag7-SKL
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236A-pcDNA3.1
Plasmid#85764Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236ADepositorInsertUSPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
UseTagsHAExpressionMammalianMutationcatalytic inactive mutant C236APromoterCMVAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-USPL1-C236S-pcDNA3.1
Plasmid#85763Purposemammalian expression of full length HA tagged USPL1, catalytic inactive variant C236SDepositorInsertUSPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
UseTagsHAExpressionMammalianMutationcatalytic inactive mutant C236SPromoterCMVAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PalmPalm-ClopHensor
Plasmid#25940DepositorInsertPalmPalm-ClopHensor
UseTagsGap20 and Strep-IIExpressionMammalianMutationN-term (20AA) of Gap-43PromoterAvailable sinceSept. 13, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2-YFP-CWT
Plasmid#176689PurposeFluorescent reporter for phagosomal escape of Staphylococcus aureusDepositorInsertcell wall-targeting domain of Lysostaphin
UseTagsYFPExpressionMammalianMutationdeletion of protase domain of LysostaphinPromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PBD-EYFP
Plasmid#13723DepositorInsertPBD-EYFP (PAK1 Human)
UseTagsEYFPExpressionMammalianMutationp21 binding domain of human Pak1(amino acids 65-1…PromoterAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_T
Plasmid#147556PurposeMammalian Expression of HsSmg7DepositorInsertHsSmg7 (SMG7 Human)
UseTagsExpressionMammalianMutationtwo silent mutation G216A, A1521G and one non sil…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-LOV-VP16
Plasmid#42499PurposeLOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factorDepositorInsertFKF1 (N-LOV, G128D mutant) fused with VP16
UseTagsF-type VP16 activation domain and HA tagExpressionMammalianMutationG128D 1-168aaPromoterCMVAvailable sinceMarch 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-2delta
Plasmid#124146PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-CIB1-Myc
Plasmid#32505DepositorInsertCIB1 (CIB1 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi1-91-NLuc
Plasmid#207148PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI1 (GNAI1 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-sACE2v2.4(732)-8h
Plasmid#154103PurposeMammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)DepositorInsertAngiotensin-converting enzyme 2 (ACE2 Human)
UseTagsShort GSG linker and 8his tagExpressionMammalianMutationExtracellular, soluble protease domain (a.a. M1-G…PromoterCMVAvailable sinceJune 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA HA FAK Y397F
Plasmid#50503DepositorInsertprotein tyrosine kinase, mutated
UseTagsHAExpressionMammalianMutationY397FPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-mCherry-N
Plasmid#210271PurposeN-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.DepositorTypeEmpty backboneUseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-ERK2 D319N
Plasmid#8979DepositorInsertERK2 (Mapk1 Rat)
UseTagsHAExpressionMammalianMutationD319NPromoterAvailable sinceOct. 11, 2005AvailabilityAcademic Institutions and Nonprofits only