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Plasmid#217803PurposeVariant CE1 construct with mSA for template recruitment and ePhi29(D169A) DNA pol, expressed from CMV or T7 promoters.DepositorInsertmSA-linker-SV40NLS-nSpCas9-BPNLS-ePhi29-BPNLS
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLSExpressionMammalianMutationnSpCas9(H840A); Phi29(D169A/M8R/V51A/M97T/G197D/E…PromoterCMV and T7Available sinceSept. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c
Plasmid#210508Purposeexpresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cellsDepositorInsertNLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c
UseTagsGB1ExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP
Plasmid#210513Purposeexpresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cellsDepositorInsertssecreted alkaline phosphatase
CMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
UseTagsMycExpressionMammalianMutationPromoterCMV and TetOAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
Plasmid#210516Purposeexpresses NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs component in mammalian cellsDepositorInsertNTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
Plasmid#210505Purposeexpresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cellsDepositorInsertNTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16
Plasmid#210506Purposeexpresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16 component in mammalian cellsDepositorInsertPDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
p2xSTAT6-Luc2P
Plasmid#35486DepositorInsertSTAT6 promoter
UseLuciferaseTagsExpressionMammalianMutationPromoter2x Stat6-c/EBPAvailable sinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
p4xSTAT6-Luc2P
Plasmid#35554DepositorInsert4xStat6 promoter
UseLuciferaseTagsExpressionMammalianMutationPromoter4xStat6Available sinceMarch 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTS2402-Tier1-PhCMV-BlastR
Plasmid#169494PurposeTier-1 vector encoding PhCMV-driven blasticidin resistance gene BlastR (PhCMV-BlastR-pA).DepositorInsertPCMV-driven blasticidin-S deaminase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS1021-Tier1-PhCMV-PuroR
Plasmid#169492PurposeTier-1 vector encoding PhCMV-driven marker for puromycin selection (PhCMV-PuroR-pA)DepositorInsertPCMV-driven puromycin N-acetyl-transferase
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2404-Tier1-PhCMV-NeoR
Plasmid#169496PurposeTier-1 vector encoding PhCMV-driven neomycin resistance gene NeoR (PhCMV-NeoR-pA).DepositorInsertPCMV-driven aminoglycoside 3'-phosphotransferase, APH 3' II
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pEF1a-PEmax-P2A-GFP
Plasmid#202000PurposeBased on the pCMV-PEmax-P2A-GFP, we replaced the CMP promoter with the EF1a promoter for expression in iPSC.DepositorInsertPEmax-P2A-GFP
UseCRISPR and Synthetic BiologyTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irC6kwh
Plasmid#44725DepositorInsertspCMV-D2i promoter
mCherry
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irG4kwh
Plasmid#44723DepositorInsertspCMV-D2i promoter
EGFP
UseSynthetic Biology; Expression regulator/reporterTagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-D2irTN6kwh
Plasmid#44724DepositorInsertspCMV-D2i promoter
htetR::NLS
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDsRed-Sensor
Plasmid#22743DepositorInsertmodified pCMV-DsRed-Express (Clontech)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pDN-D2irTNG4kwh
Plasmid#44722DepositorInsertspCMV-D2i promoter
htetR::NLS::EGFP
UseSynthetic Biology; Expression regulator/reporter;…TagsRabbit β-globin intron II and WPREExpressionMammalianMutationInitiator motif (Inr) displaced relative to pCMV-…PromoterpCMV-D2iAvailable sinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only