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Plasmid#164080PurposeTo express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansionDepositorInsertMtPylRS-478(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MfBulKRS (human-opti)
Plasmid#164196PurposeTo express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansionDepositorInsertMf BulKRS(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationY269A;Y347FPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
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MDH1-miR-181a1-Dusp6
Plasmid#17849DepositorInsertDusp6-EGFP (Dusp6 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 29, 2008AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Climp63(1–336)
Plasmid#186957PurposeExpresses mCherry-Climp63 with partial luminal domainDepositorInsertCkap4 (Ckap4 Mouse)
UseTagsmCherryExpressionMammalianMutationOnly remain partial luminal domainPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Climp63(1–301)
Plasmid#186956PurposeExpresses mCherry-Climp63 with partial luminal domainDepositorInsertCkap4 (Ckap4 Mouse)
UseTagsmCherryExpressionMammalianMutationOnly remain partial luminal domainPromoterCMV PromoterAvailable sinceSept. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Climp63(1–437)
Plasmid#186958PurposeExpresses mCherry-Climp63 with partial luminal domainDepositorInsertCkap4 (Ckap4 Mouse)
UseTagsmCherryExpressionMammalianMutationOnly remain partial luminal domainPromoterCMV PromoterAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
alpha1Ddelta-G12-FRB
Plasmid#87454Purposerapamycin-mediated heterodimerization between IQV and PCRD/DCRD from C-terminal of LTCCsDepositorInserta1D short variants (Cacna1d Rat)
UseTagsFRBExpressionMammalianMutationdelete KYPAKNTTIALQMLERMLPromoterCMVAvailable sinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:myc-GluA1 TKIT donor
Plasmid#169419PurposeDonor DNA for TKIT knockin of Myc to GluA1 in mouseDepositorInsertMyc (Myc Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _701-902
Plasmid#86821Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM2-rPMCA3b
Plasmid#47763Purposemammalian expression of rat PMCA3bDepositorInsertPMCA3b (Atp2b3 Rat)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 delta 610-615
Plasmid#19941DepositorInsertcullin 1 delta 610-615 (CUL1 Human)
UseTagsHAExpressionMammalianMutationCUL1 with a ROC1 binding mutantPromoterAvailable sinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLux02
Plasmid#78282PurposemRFP1 expressed behind an AHL-inducible constructDepositorInsertAHL inducible mRFP1
UseTagsExpressionMutationPromoterAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _462-699
Plasmid#86819Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _568-637
Plasmid#86820Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-CUL1 L756A/I757A
Plasmid#19938DepositorInsertcullin 1 L756A/I757A (CUL1 Human)
UseTagsHA2ExpressionMammalianMutationMutation of two adjacent hydrophobic residues, L…PromoterAvailable sinceApril 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Hyg-FHIT
Plasmid#16410DepositorInsertFHIT Hyg targeting (FHIT Human)
UseAAV; Adeno-associated viralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRAN357
Plasmid#120898PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target proteinDepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro human Frataxin
Plasmid#11505DepositorInsertFrataxin (FXN Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only