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Plasmid#146702PurposeInsect Expression of DmNot1_1710-2505DepositorInsertDmNot1_1710-2505 (Not1 Fly)
UseTagsExpressionInsectMutationtwo non silent mutations (A1747T, G1999S) and one…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup-Y342AL347A_O
Plasmid#147103PurposeInsect Expression of DmCup-Y342AL347ADepositorInsertDmCup-Y342AL347A (cup Fly)
UseTagsExpressionInsectMutationY342A, L347A, I350L, P418S, K1045NPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-dsRNAres_J
Plasmid#146692PurposeInsect Expression of DmHPat-dsRNAresDepositorInsertDmHPat-dsRNAres (Patr-1 Fly)
UseTagsExpressionInsectMutationtwo non silent, A479V, G568D and one silent mutat…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-D207A-dsRNAres-V5His6_O
Plasmid#147065PurposeInsect Expression of DmXRN1-D207A-dsRNAresDepositorInsertDmXRN1-D207A-dsRNAres (pcm Fly)
UseTagsExpressionInsectMutationone silent mtuation compared to the sequence give…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-del1323-1355-dsRNAres-V5His6_N
Plasmid#146969PurposeInsect Expression of DmXRN1-del1323-1355-dsRNAresDepositorInsertDmXRN1-del1323-1355-dsRNAres (pcm Fly)
UseTagsExpressionInsectMutationone silent mtuation compared to the sequence give…PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delCterm_E
Plasmid#146252PurposeInsect Expression of DmGW182-delC-termDepositorInsertDmGW182-delC-term (gw Fly)
UseTagsExpressionInsectMutationV758A, Q817R, and N853SPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E
Plasmid#146241PurposeInsect Expression of DmSMG6-siRNAresDepositorInsertDmSMG6-siRNAres (Smg6 Fly)
UseTagsExpressionInsectMutationT151A, deleted A947PromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmGe1_1220-1354_G
Plasmid#146420PurposeBacterial Expression of DmGe1_1220-1354DepositorInsertDmGe1_1220-1354 (Ge-1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-NusA-DmEDC3_331-440_H
Plasmid#146448PurposeBacterial Expression of DmEDC3_331-440DepositorInsertDmEDC3_331-440 (Edc3 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-G2304D
Plasmid#182845Purposemutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sitesDepositorInsertTRR 2011-2431 (trr Fly)
UseTagsGSTExpressionBacterialMutationmutation G2304D (GGC/GAC)PromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1-287)
Plasmid#182839PurposeCBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)DepositorInsertCBP1-287
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-UTX(420-633)-H6
Plasmid#183773PurposeExpress pET-UTX(420-633)-H6 in E.coli. Purified pET-UTX(420-633)-H6 was used for generating antibodies in rabbits.DepositorInsertUTX
UseTags6 His tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-228)-H6
Plasmid#183779PurposeExpress PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.DepositorInsertPolycomb (Pc Fly)
UseTags6 His Tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-390)-H6
Plasmid#183781PurposeExpress PC(75-390)-H6 in E. coli. PC(75-390)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (w Fly)
UseTags6 His tag at the C-terminusExpressionBacterialMutation6 His tag at the C-terminusPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-PC(1-86)-H6
Plasmid#183780PurposeExpress PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assaysDepositorInsertPolycomb (Pc Fly)
UseTags6 His Tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-SU(Z)12(624-900)-H6
Plasmid#183772PurposeExpress of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.DepositorInsertSU(Z)12 residues 624-900 (Su(z)12 Fly)
UseTagsHis tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3(K27Q)
Plasmid#182843Purposea K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
UseTagsGSTExpressionInsectMutationPromoterActin5CAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3
Plasmid#182842PurposeA PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
UseTagsGSTExpressionInsectMutationPromoterActin5CAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1603-2678)
Plasmid#183774PurposeExpress GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.DepositorInsertnej (nej Fly)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRX-C751-E3616S
Plasmid#182844Purposemutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG inductionDepositorInsertTRX (w Fly)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only