-
Plasmid#46918PurposeHuman pSico-based U6 vector containing murine U6 promoter and sgRNA targeting endogenous CD71 geneDepositorInsertsUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMV and U6Available sinceOct. 1, 2013AvailabilityAcademic Institutions and Nonprofits only
-
pVC-FUS-R521C
Plasmid#175067Purposeexpresses mVenus and mCherry tagged to mutant of the FUS (R521C) protein in mammalian cellsDepositorInsertFUS glycine rich protein (FUS Human)
UseTagsHA and mVenus + mCherryExpressionMammalianMutationchanged Arginin 521 to Cystein; mVenus: C-Termina…PromoterCMV PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-YAPS94A
Plasmid#84010Purposeexpresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationS94APromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
ABE8.17-m
Plasmid#136298Purposeexpresses ABE8.17-m in mammalian cellsDepositorInsertABE8.17-m
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Mfn2-HA S27A
Plasmid#139193PurposeExpresses C-terminal HA tagged human Mfn2 S27A in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags3HAExpressionMammalianMutationSerine 27 to AlaninePromoterCMV promoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
-
-
pcDNA3.1 Mfn2-HA S27D
Plasmid#139194PurposeExpresses C-terminal HA tagged human Mfn2 S27D in mammalian cellsDepositorInsertMFN2 (MFN2 Human)
UseTags2HAExpressionMammalianMutationSerine 27 to Aspartic AcidPromoterCMV promoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
-
-
pAAV-STAT3
Plasmid#195575PurposeExpresses STAT3DepositorInsertSignal transducer and activator of transcription 3 (Stat3 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterCMV enhancer and promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.27_eSOX17
Plasmid#195498PurposeFirefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17DepositorInsertFull distal endoderm enhancer region of SOX17 (SOX17 Human)
UseTagsminP_luc2-hPESTExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4.27_eSOX17.2
Plasmid#195499PurposeFirefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17DepositorInsertCore distal endoderm enhancer region of SOX17 (SOX17 Human)
UseTagsminP_luc2-hPESTExpressionMammalianMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBrain-ch-TOGKDP-GFP-shch-TOG
Plasmid#69113PurposeDual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.DepositorInsertsUseRNAiTagsEGFPExpressionMammalianMutationSilent mutations to confer shRNA resistance.PromoterCMVAvailable sinceOct. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
FcD265A-FLAG
Plasmid#65420PurposeMammalian expression plasmid for murine Fc-FLAG, used for monomeric Fc-IL2 productionDepositorInsertFcD265A-FLAG
UseTagsFLAGExpressionMammalianMutationD265APromoterModified CMV PromoterAvailable sinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-CES2A-ΔC-S227A
Plasmid#200556PurposeMammalian expression of secreted mouse CES2A S227A mutantDepositorInsertMouse Carboxylesterase 2A S227A mutant delta C-terminal (Ces2a Mouse)
UseTags6xHis, Flag, and V5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-CES2A-ΔC
Plasmid#200554PurposeMammalian expression of secreted mouse CES2ADepositorInsertMouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)
UseTags6xHis, Flag, and V5ExpressionMammalianMutationPromoterCMV promoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
ABE8.17-d
Plasmid#136299Purposeexpresses ABE8.17-d in mammalian cellsDepositorInsertABE8.20-d
UseTagsC-terminal BPNLSExpressionMammalianMutationD10A in S. pyogenes Cas9, TadA mutations describe…PromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW TetO HA-AMPKa2 WT
Plasmid#69826PurposeExpresses AMPK alpha 1 in mammalian cells in a doxycycline inducible mannerDepositorInsert5'-AMP-activated protein kinase catalytic subunit alpha-2 (PRKAA2 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterminimal CMV promoter with tetracycline operatorAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS0045
Plasmid#131124PurposeMammalian Target-ACE expression vectorDepositorInsertTadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1, TadA, and UGIExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219V …PromoterCMV promoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only