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Anti-MMACHC [N230/21R]

Plasmid

#114555

Purpose

Mammalian Expression Plasmid of anti-MMACHC (Human). Derived from hybridoma N230/21.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-MMACHC (Homo sapiens) recombinant mouse monoclonal antibody (MMACHC Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)

Plasmid

#104570

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x3)

Use

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ10878

Plasmid

#183964

Purpose

CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Insert

HyperdCas12a

Use

CRISPR

Tags

HA, Hygromycin Resistance, and mCherry

Expression

Mammalian

Mutation

D156R, E292R, D235R, D350R

Promoter

CAG

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

Plasmid

#214885

Purpose

Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ10873

Plasmid

#183961

Purpose

U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

Depositor

Stanley Qi

Article

Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

Inserts

HyperdCas12a
poly-crRNA array targeting Sox2-Klf4-Oct4

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D832A, D156R, E292R, D235R, D350R

Promoter

CAG and U6

Available since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCAS

Plasmid

#60847

Purpose

Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast

Depositor

Jamie Cate

Article

Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife

Inserts

S. pyogenese Cas9
RNA pol III promoter (tRNA-Tyr)
hepatitis delta virus ribozyme, genomic
sgRNA

Use

CRISPR and Synthetic Biology

Tags

NLS/His8 and TTT 3' extension prior to sgRNA

Expression

Bacterial and Yeast

Mutation

L4 is UUCG tetraloop and guide targets LYP1 (CATA…

Promoter

Available since

Jan. 13, 2015

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_1_001

Plasmid

#167347

Purpose

ddPCR gating control for droplets containing all 5 targets (2 in pol)

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_9_003

Plasmid

#167349

Purpose

ddPCR gating control for droplets containing either gag+env targets OR tat + env targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gag_tat_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_6_002

Plasmid

#167348

Purpose

ddPCR gating control for droplets containing either gag+5'pol targets OR 3'pol+tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_gag_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_29_006

Plasmid

#167352

Purpose

ddPCR gating control for droplets containing either env+5'pol targets OR env+3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol_env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Seattle_IPDA_control_14_004

Plasmid

#167350

Purpose

ddPCR gating control for droplets containing the env target

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

env

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_32_007

Plasmid

#167353

Purpose

ddPCR gating control for droplets containing 5'pol or 3'pol targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

pol

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Seattle_IPDA_control_17_005

Plasmid

#167351

Purpose

ddPCR gating control for droplets containing gat and tat targets

Depositor

Florian Hladik

Article

Levy et al Cell Rep Med. 2021 Apr 12;2(4):100243. d

Insert

gat_tat

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pB-Gaolf-Integration

Plasmid

#129456

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

G alpha olf (Gnal Mouse)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

TRE (Tet-On)

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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CCR7-DuET

Plasmid

#213203

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR7 (CCR7 Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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CCR2-DuET

Plasmid

#213198

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR2 (CCR2 Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CCR8-DuET

Plasmid

#213204

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR8 (CCR8 Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
CCR5-DuET

Plasmid

#213201

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR5 (CCR5 Human)

Use

Tags

1D4 and FLAG

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
8xNFAT-ZsG-hCD8

Plasmid

#153417

Purpose

NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.V2

Plasmid

#127848

Purpose

non-standard AAV2 rep-AAV-PHP.V2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V2 VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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