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pRK5-HA-SH3BP4 W92A

Plasmid

#46893

Purpose

expresses HA tagged SH3BP4 W92A mutant in mammalian cells

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 W92A (SH3BP4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Promoter

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pFP121

Plasmid

#21670

Depositor

James Haber

Article

Pâques et al Mol Cell Biol. 1997 Nov . 17(11):6765-71

Insert

Lacz (lacZ E. coli)

Use

Centromeric plasmid

Tags

Expression

Mutation

The second lacZ sequence has been modified, the E…

Promoter

Available since

Sept. 8, 2009

Availability

Academic Institutions and Nonprofits only
pLyn-CaRQ

Plasmid

#34559

Depositor

Kevin Truong

Article

Mills et al Chem Biol. 2011 Dec 23;18(12):1611-9.

Insert

pLyn-CaRQ (RHOA Human)

Use

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L in RhoA

Promoter

CMV, T7

Available since

March 29, 2012

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-C373T

Plasmid

#24734

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

C replaced by T at position 373 resulting in Q125…

Promoter

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pDONR221-hSOX10-C1271T

Plasmid

#24736

Depositor

William Pavan

Article

Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

Insert

(sex determining region Y)-box 10 (SOX10 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

C replaced with T at position 1271 resulting in A…

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only
pACB108

Plasmid

#222207

Purpose

For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with a C-terminal His tag

Depositor

Gregg Beckham

Article

Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.

Insert

fghA

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Xlone Puro SNAP-eGFP-BASU

Plasmid

#218452

Purpose

expresses the SNAP-eGFP-BASU fusion protein via stable PiggyBac integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

SNAP-eGFP-BASU

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3GS

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5_NLS-Halo-eGFP-BASU

Plasmid

#218449

Purpose

expressed the NLS-Halo-eGFP-BASU fusion protein via stable Flp-In integration

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

NLS-Halo-eGFP-BASU

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrA

Plasmid

#216229

Purpose

S. aureus USA300 GyrA Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET151-GyrB

Plasmid

#216233

Purpose

S. aureus USA300 GyrB Codon-optimized for E. coli

Depositor

Eric Brown

Article

French et al Cell Rep. 2024 Apr 4;43(4):114053. doi:

Insert

DNA gyrase

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Drosophila Wdr20-V5

Plasmid

#217622

Purpose

Mammalian expression of V5 tagged Drosophila Wdr20

Depositor

Ethan Lee

Article

Spencer et al Nat Commun. 2023 Oct 5;14(1):6174. doi:

Insert

WDR20

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pACYC Destination

Plasmid

#207675

Purpose

Trio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).

Depositor

Ramon Gonzalez

Article

Lee et al Metab Eng. 2024 Mar;82:262-273. doi: 10.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

CymRC, PhlF, VanCC

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFP-N

Plasmid

#210270

Purpose

N-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-p62 var2 PB1mut

Plasmid

#204578

Purpose

Expresses 3xFLAG-p62 var2 PB1mut in mammalian cells

Depositor

Masaaki Komatsu

Article

Kageyama et al Mol Cell Biol. 2018 Jan 16. pii: MCB.006

Insert

p62 (Sqstm1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

K7A/D69A

Promoter

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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GFP SMG1 T3550A/S3556A

Plasmid

#199572

Purpose

Expresses mutated SMG1 fused to GFP

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

SMG1 (SMG1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

T3550A and S3556A

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-HsSmg7-K66ER163E_S

Plasmid

#147479

Purpose

Mammalian Expression of HsSmg7-K66ER163E

Depositor

Elisa Izaurralde

Article

Fukuhara et al Mol Cell. 2005 Feb 18;17(4):537-47. doi:

Insert

HsSmg7-K66ER163E (SMG7 Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation G2560A (V854I) and three …

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRRP12-EBMmut_K

Plasmid

#146767

Purpose

Mammalian Expression of HsRRP12-EBMmut

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsRRP12-EBMmut (RRP12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM41P-HsUPF3b-iso2_145-470_K

Plasmid

#146754

Purpose

Bacterial Expression of HsUPF3biso2_145-470

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF3biso2_145-470 (UPF3B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsSmg6_1-207_K

Plasmid

#146755

Purpose

Bacterial Expression of HsSmg6_1-207

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsSmg6_1-207 (SMG6 Human)

Use

Tags

Expression

Bacterial

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-HsUPF3b-iso2_145-470_K

Plasmid

#146759

Purpose

Bacterial Expression of HsUPF3biso2_145-470

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF3biso2_145-470 (UPF3B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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