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pHH0103_ESR1_Hormone-recep

Plasmid

#109906

Purpose

Protein expression and purification of ESR1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESR1_Hormone-recep (ESR1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-C/EBPbZIP (pGEX-bZIPa)

Plasmid

#12435

Depositor

Dong-Er Zhang

Article

Zhang et al Mol Cell Biol. 1996 Mar . 16(3):1231-40.

Insert

68 a.a. fragment of C/EBP alpha (CEBPA Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pHH0103_EPB41L3_FERM

Plasmid

#110011

Purpose

Protein expression and purification of EPB41L3_FERM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EPB41L3_FERM (EPB41L3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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cmv hTid Short H121Q

Plasmid

#13710

Depositor

Karl Munger

Article

Schilling et al Virology. 1998 Jul 20. 247(1):74-85.

Insert

hTid Short H121Q (DNAJA3 hTid Short H121Q, Human)

Use

Tags

Expression

Mammalian

Mutation

H121Q

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
RH2b-bio

Plasmid

#47778

Purpose

Expresses enzymatically monobiotinylated partial RH2b ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised RH2b (partial)

Use

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pHH0103_MAPK3_Pkinase

Plasmid

#110023

Purpose

Protein expression and purification of MAPK3_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

MAPK3_Pkinase (MAPK3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_AP2M1_Adap-comp-sub

Plasmid

#109855

Purpose

Protein expression and purification of AP2M1_Adap-comp-sub

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

AP2M1_Adap-comp-sub (AP2M1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (1-250) (P#467)

Plasmid

#14606

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-250) (CCNT1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-250

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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ZIKV_NASBA_32B

Plasmid

#75011

Purpose

Portion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sites

Depositor

Alexander Green, James Collins

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_32B

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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mCardinal-CytERM-N-17

Plasmid

#56158

Purpose

Localization: Endoplasmic Reticulum, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

CytERM

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
ZIKV_NASBA_27B

Plasmid

#75010

Purpose

Portion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sites

Depositor

Alexander Green, James Collins

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_NASBA_27B

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA5_FRB-pBirA-HA

Plasmid

#83415

Purpose

proximity-directed biotin labeling on the FRB domain of mTOR in mammalian cell

Depositor

Hyun-Woo Rhee

Article

Lee et al ACS Cent Sci. 2016 Aug 24;2(8):506-16. d

Insert

FRB-pBirA-HA (MTOR Human, Synthetic)

Use

Tags

HA and pBirA

Expression

Mammalian

Mutation

Promoter

CMV-F

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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Pf92-bio

Plasmid

#47728

Purpose

Expresses enzymatically monobiotinylated full-length Pf92 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

Depositor

Gavin Wright

Article

Crosnier et al Mol Cell Proteomics. 2013 Sep 16.

Insert

Codon-optimised Pf92

Use

Tags

enzymatic biotinylation sequence and rat Cd4 d3+4

Expression

Mammalian

Mutation

Exogenous signal peptide of mouse origin, changed…

Promoter

CMV

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_GNAO1_G-alpha

Plasmid

#110013

Purpose

Protein expression and purification of GNAO1_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAO1_G-alpha (GNAO1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_CASP2_Peptidase-C14

Plasmid

#109996

Purpose

Protein expression and purification of CASP2_Peptidase-C14

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CASP2_Peptidase-C14 (CASP2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_HLA-DRB1_MHC-II-beta

Plasmid

#110021

Purpose

Protein expression and purification of HLA-DRB1_MHC-II-beta

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HLA-DRB1_MHC-II-beta (HLA-DRB1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-mouseAtoh1-IRES-GFP

Plasmid

#73572

Purpose

Mammalian retroviral expression of Atoh1

Depositor

Martine Roussel

Article

Vo et al Cancer Cell. 2016 Jan 11;29(1):5-16. doi

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only
pHH0103_GNA13_G-alpha

Plasmid

#109913

Purpose

Protein expression and purification of GNA13_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNA13_G-alpha (GNA13 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM24-C840W shRescue

Plasmid

#28137

Depositor

Michelle Barton

Article

Tsai et al Nature. 2010 Dec 16. 468(7326):927-32.

Insert

TRIM24 (TRIM24 Human)

Use

Tags

FLAGX2

Expression

Mammalian

Mutation

C840Wmutated at aa 308 and 309 for shRNA rescue; …

Promoter

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pHH0103_ESR2_Hormone-recep

Plasmid

#109907

Purpose

Protein expression and purification of ESR2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESR2_Hormone-recep (ESR2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge