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Showing: 4521 - 4560 of 148587 results

  1. pX459-sgAAVS1

    Plasmid
    #184403
    Purpose
    pX459 (sgRNA and Cas9 expressing plasmid, RRID:Addgene_62988) with targeting sequence specific to AAVS1; targeting sequence is: GGGGCCACTAGGGACAGGAT
    Depositor
    Robert Bradley
    Article
    Udy et al Life Sci Alliance. 2021 Dec 8;5(3). pii:
    Insert
    AAVS1-specific sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    184403-plasmid-map-sequence-id-362381

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  2. pSLQ10873

    Plasmid
    #183961
    Purpose
    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR
    Depositor
    Stanley Qi
    Article
    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d
    Inserts
    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    Use
    CRISPR
    Tags
    HA
    Expression
    Mammalian
    Mutation
    D832A, D156R, E292R, D235R, D350R
    Promoter
    CAG and U6
    Available since
    Aug. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    183961-plasmid-map-sequence-id-369669

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  4. Fragment1 with TLS2

    Plasmid
    #196983
    Purpose
    Gateway-compatible gRNA backbone with TLS2 mobility signal. Template for the addition of target sequences to produce 2x graft-mobile gRNA-TLS2.
    Depositor
    Friedrich Kragler
    Article
    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    Intermediate fragment for assembly of gRNA-TLS2 construct
    Use
    Gateway-compatible entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196983-plasmid-map-sequence-id-390457

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  9. pCBC-DT3T4

    Plasmid
    #50592
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T3, gRNA scaffold, U6-1tplus, U6-26p, T4
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50592-plasmid-map-sequence-id-372989

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  13. pcDNA3-Clover

    Plasmid
    #40259
    Purpose
    GFP with robust performance in protein fusions and useful as FRET acceptor for mRuby2 in a FRET pair that offers bright fluorescence, dynamic range, and photostability while limiting emissions overlap
    Depositor
    Michael Lin
    Article
    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm
    Has Service
    Cloning Grade DNA
    Insert
    Clover GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE
    Available since
    Sept. 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    40259-plasmid-map-sequence-id-215680

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  15. MSCV-Cre

    Plasmid
    #24064
    Depositor
    Dan Littman
    Insert
    Cre
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 27, 2010
    Availability
    Academic Institutions and Nonprofits only
    24064-plasmid-map-sequence-id-322907

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  16. R777-E139 Hs.NF1

    Plasmid
    #70423
    Purpose
    Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    NF1 (NF1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70423-plasmid-map-sequence-id-125872

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  17. pMN-334

    Plasmid
    #195468
    Purpose
    Lentiviral Integratable Inducible synZiFTR ZF10 + Reporter
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    inverted[8X ZF10 BS - ybTATA - mCherry] - pSFFV - ZF10 - NS3 - p65
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195468-plasmid-map-sequence-id-386007

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  23. PB-TET

    Plasmid
    #20909
    Purpose
    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)
    Depositor
    Andras Nagy
    Article
    Woltjen et al Nature. 2009 Mar 1. ():.
    Insert
    Gateway Destination Vector
    Use
    piggybac
    Tags
    IRES-bGeo
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    20909-plasmid-map-sequence-id-174009

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  25. pcDNA3-TLR4-YFP

    Plasmid
    #13018
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 4 (TLR4 Human)
    Use
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13018-plasmid-map-sequence-id-339915

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  28. pMIA10.53-Clover

    Plasmid
    #214450
    Purpose
    BxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.
    Depositor
    Roger Foo
    Article
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Insert
    mClover
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214450-plasmid-map-sequence-id-431690

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  30. pMIA10.5-Clover

    Plasmid
    #214449
    Purpose
    BxbI landing-pad mClover donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.
    Depositor
    Roger Foo
    Article
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Insert
    mClover
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214449-plasmid-map-sequence-id-431685

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  36. pSBbi-Neo

    Plasmid
    #60525
    Purpose
    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene
    Depositor
    Eric Kowarz
    Article
    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b
    Type
    Empty backbone
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60525-plasmid-map-sequence-id-97521

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  38. NL4-3 mCherry Luciferase

    Plasmid
    #44965
    Purpose
    HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.
    Depositor
    Warner Greene
    Article
    Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371
    Insert
    mCherry, Luciferase, IRES
    Use
    Lentiviral
    Tags
    Luciferase, IRES and mCherry with D212G
    Expression
    Mutation
    S34C mutation in Nef
    Promoter
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    44965-plasmid-map-sequence-id-352462

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Showing: 4521 - 4560 of 148587 results