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pAV-CAG-Sun1-GFP-WPRE-BC5-pA

Plasmid

#220757

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

Sun1-GFP-WPRE-BC5

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-BC1-pA

Plasmid

#220753

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

Sun1-GFP-WPRE-BC1

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-BC11-pA

Plasmid

#220763

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

Sun1-GFP-WPRE-BC11

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223_NKX2-1_HD_160-223AA

Plasmid

#221477

Purpose

Gateway cloning of NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Promoter

Available since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD-Y115S/Y132S/Y139S

Plasmid

#216408

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

Depositor

Nicolas Fawzi

Article

Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

Insert

Efg1-ΔDBD-Y115S/Y132S/Y139S

Use

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed Tyrosine 115, 132 and 139 to Serine, and …

Promoter

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_HD_160-223AA

Plasmid

#221489

Purpose

NKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Deletion of 1-159AA N-terminal and 224-371AA C-te…

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-OPTN

Plasmid

#208866

Purpose

Stably express GFP-tagged OPTN for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

OPTN (OPTN Human)

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC9-pA

Plasmid

#220773

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

oScarlert-WPRE-BC9

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

June 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-BC3-pA

Plasmid

#220755

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

Sun1-GFP-WPRE-BC3

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC2-pA

Plasmid

#220766

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

oScarlert-WPRE-BC2

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-oScarlet-WPRE-BC3-pA

Plasmid

#220767

Purpose

Expression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

oScarlert-WPRE-BC3

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1-Nir3-LNS2

Plasmid

#220087

Purpose

C-terminal PA binding domain of Nir3

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PITPNM2 (PITPNM2 Human)

Use

Tags

mEGFP-GGGGSHM

Expression

Mammalian

Mutation

amino acids 925-1209

Promoter

CMV

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pmiRFP670-C1-Nir1-LNS2

Plasmid

#220085

Purpose

high affinity PA biosensor with far-red fluorophore

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PITPNM3 (PITPNM3 Human)

Use

Tags

iRFP670-GGSGGM

Expression

Mammalian

Mutation

amino acids 613-897

Promoter

CMV

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-CAG-Sun1-GFP-WPRE-BC7-pA

Plasmid

#220759

Purpose

Expression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracing

Depositor

Vijay Samineni

Article

Yang et al bioRxiv 2024.04.24.590975

Insert

Sun1-GFP-WPRE-BC7

Use

AAV

Tags

Expression

Mutation

Promoter

CAG

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pSCL475

Plasmid

#219503

Purpose

Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

Insert

Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL7

Available since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker

Plasmid

#207172

Purpose

expression plasmid for Gal4DBD-HIF-CAD expression GalO linker

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD GalO linker

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tet2puro-gal4DBD-HIFCAD

Plasmid

#207173

Purpose

Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD

Use

Lentiviral; Dox inducible

Tags

2x Myc

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT (PGK) 12xHRE-NanoLuc

Plasmid

#208575

Purpose

hypoxic response element driven NanoLuc Lentiviral reporter

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

NanoLuciferase
d2eGFP
12xHRE

Use

Lentiviral, Luciferase, and Synthetic Biology

Tags

3x SV40 NLS and PEST

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-SINTBAD

Plasmid

#216840

Purpose

Stably express FKBP-GFP-tagged SINTBAD for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

SINTBAD (TBKBP1 Human)

Use

Lentiviral

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-NAP1-L226Q/L233Q (delta-TBK1)

Plasmid

#208869

Purpose

For mammalian expression of NAP1 (delta-TBK1)

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

NAP1 (NAP1L1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

L226Q/L233Q (disrupts TBK1 binding)

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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