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pInducer-myc-ERK1-R84S/S170D/Y280A

Plasmid

#213596

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/Y280A cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, Y280A

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Pan-GRK [N145/20R]

Plasmid

#206536

Purpose

Mammalian Expression Plasmid of anti-Pan-GRK (Mouse). Derived from hybridoma N145/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Pan-GRK (Mus musculus) recombinant Mouse monoclonal antibody (Grk5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-Myr-DnaJB1(H33Q)-PKAcat-mCherry

Plasmid

#181855

Purpose

mCherry-tagged DnaJB1-PKA catalytic subunit fusion N-terminal myristoylation site and H33Q DnaJB1 mutation to block Hsp70 binding; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Histidine 33 in DnaJB1 changed to glutamine.

Promoter

CMV

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT3-M339I

Plasmid

#116105

Purpose

Lentiviral expression of AKT3 M339I

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Tags

Expression

Mutation

M339I

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-AKT3-S370P

Plasmid

#116106

Purpose

Lentiviral expression of AKT3 S370P

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT3 (AKT3 Human)

Use

Lentiviral

Tags

Expression

Mutation

S370P

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.V201I

Plasmid

#81453

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

V201I; p.117delE

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT1_p.R370C

Plasmid

#81416

Purpose

Gateway Donor vector containing AKT1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT1 (AKT1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R370C; p.114delE

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_AKT3_p.E17K

Plasmid

#82172

Purpose

Gateway Donor vector containing AKT3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

AKT3 (AKT3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E17K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223-AKT1

Plasmid

#23752

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT1 (AKT1 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag AKT3

Plasmid

#20423

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AKT3 (AKT3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223-AKT3

Plasmid

#23539

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

AKT3 (AKT3 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

S2R

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only