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pCDB327

Plasmid

#113671

Purpose

expresses Ulp1_R3 in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Ulp1_R3

Use

Tags

6-His

Expression

Bacterial

Mutation

contains solubility enhancing mutations

Promoter

T7

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCCL-CellCycle

Plasmid

#132429

Purpose

Expressing Fucci cell cycle indicator in mammalian cells

Depositor

John M. Greally

Article

Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

Insert

hCdt, hGeminin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLX311-Cas9

Plasmid

#118018

Purpose

Constitutive expression of Cas9

Depositor

David Root, William Hahn

Article

Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

Insert

Cas9

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-smURFP

Plasmid

#80349

Purpose

Lentiviral transfer vector under the CMV promoter that expresses smURFP.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Insert

smURFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pYD1

Plasmid

#73447

Purpose

expression vector designed for expression, secretion, and display of proteins on the extracellular surface of Saccharomyces cerevisiae cells

Depositor

Dane Wittrup

Article

Kieke et al Protein Eng. 1997 Nov;10(11):1303-10.

Type

Empty backbone

Use

Tags

6xHis, AGA2, T7 peptide, V5 tag, and Xpress tag

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pU6-Sp-pegRNA-RNF2_+5GtoT

Plasmid

#135957

Purpose

S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_5GtoT pegRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pTNS1

Plasmid

#64967

Purpose

Tn7 transposase expression

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

tnsABCD

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 23, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEGFP-Lifeact-7

Plasmid

#54610

Purpose

Localization: Actin, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

mEGFP-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pFLAG AXL

Plasmid

#105933

Purpose

Mammalian expression of Flag-tagged AXL

Depositor

Rosa Marina Melillo

Article

Krishnamoorthy et al J Biol Chem. 2013 Jun 14;288(24):17481-9

Insert

AXL (AXL Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE TRE dCas9-VP64

Plasmid

#50916

Purpose

Tet-regulatable dCas9-VP64 2nd generation lentiviral expression vector

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

3xHA and VP64

Expression

Mammalian

Mutation

D10A, H840A nuclease-deficient

Promoter

TRE

Available since

March 18, 2014

Availability

Academic Institutions and Nonprofits only

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p65-mNeonGreen

Plasmid

#127172

Purpose

Fluorescently tagged p65 reporter

Depositor

Paul Blainey

Article

Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

Insert

GFP-RelA (RELA Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-p65

Plasmid

#111190

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Schmid et al J Biol Chem. 2000 Jun 2;275(22):17035-42

Insert

RELA (RELA Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-GFP-LaminA-IRES-Hygromycin

Plasmid

#134867

Purpose

Expresses EGFP-tagged nuclear envelope marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Lamin A (LMNA Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-EEA1

Plasmid

#176491

Purpose

For lentiviral transduction of 3FLAG-EEA1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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PCV-Cas9

Plasmid

#123643

Purpose

Expresses Cas9 with an N-terminal HUH-tag called PCV2

Depositor

Wendy Gordon

Article

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Use

Tags

(EAAAK)8 linker and His6 + SUMO

Expression

Bacterial

Mutation

2-116

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3G-p300-dCas9-P2A-mCherry

Plasmid

#138456

Purpose

2nd Generation Lentiviral vector. Expresses an N-terminal p300-dCas9 fusion protein and mCherry

Depositor

Jian Xu

Article

Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

Insert

p300 core

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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circRNA-synIRES-R25-NanoLuc

Plasmid

#188116

Purpose

Template plasmid to transcribe circular RNA encoding NanoLuc

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

NanoLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAF0033 Ef1a-Pa01-2a-EGFP

Plasmid

#193460

Purpose

Expression of Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Use

Tags

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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EJ2GFP-puro

Plasmid

#44025

Depositor

Jeremy Stark

Article

Bennardo et al PLoS Genet. 2008 Jun 27;4(6):e1000110. d

Insert

EJ2GFP egfp-based chromosomal break reporter

Use

Tags

Expression

Mammalian

Mutation

insertion of I-SceI, 3 frame stop, and flanking m…

Promoter

pCAGGS

Available since

May 3, 2013

Availability

Academic Institutions and Nonprofits only

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