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N-His-GvpC-ssrA

Plasmid

#153297

Purpose

Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)

Use

Tags

His-tag and ssrA tag

Expression

Bacterial

Mutation

1) N-terminal 6xHis tag flanked by 1x glycine res…

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pTD-C_sfYFPTwinStrep

Plasmid

#45943

Purpose

expresses P.putida codon optimized super-folder sfYFP-Twin-Strep fusion protein

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Insert

synthetic sfYFP (codon usage adapted to P.putida KT2440)

Use

Tags

Twin-Strep-tag

Expression

Bacterial

Mutation

Promoter

lacIq/Ptrc

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-SOX4

Plasmid

#110367

Purpose

Mammalian expression of HA-tagged SOX4

Depositor

Carlos Moreno

Insert

SOX4 (SOX4 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pFA6a-A1:hphMX4

Plasmid

#134425

Purpose

PCR-based C-terminal A1 tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

A1

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-S6:hphMX4

Plasmid

#134433

Purpose

PCR-based C-terminal S6 tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

S6

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-SRS:hphMX4

Plasmid

#134441

Purpose

PCR-based C-terminal SRS tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-SRS:kanMX6

Plasmid

#134442

Purpose

PCR-based C-terminal SRS tag

Depositor

Robert Tomko

Article

Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

Insert

SRS

Use

Pcr-based tagging in yeast

Tags

Expression

Mutation

Promoter

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pFA6a-GFP-ura4MX6

Plasmid

#33140

Purpose

genomic GFP tagging in yeast

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Inserts

ura4MX6
GFP

Use

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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MAP3K15-TAP

Plasmid

#69728

Purpose

Express tandem tagged MAP3K15 in cells

Depositor

Daniel Liebler

Insert

Apoptosis signal-regulating kinase 3 (MAP3K15 Human)

Use

Tags

HA-Myc tag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pFETCh_ATF4

Plasmid

#72350

Purpose

Donor vector for 3' FLAG tag of human ATF4

Depositor

Richard M. Myers, Eric Mendenhall

Insert

ATF4 Homology Arms (ATF4 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTubulin-BC2T

Plasmid

#108970

Purpose

expresses tubulin-BC2-tag in mammalian cells

Depositor

Ulrich Rothbauer

Article

Virant et al Nat Commun. 2018 Mar 2;9(1):930. doi: 10

Insert

a-tubulin

Use

Tags

BC2-tag

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pTDpelB-C_sfYFPTwinStrep

Plasmid

#45944

Purpose

expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Article

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Insert

synthetic sfYFP (codon usage adapted to P.putida KT2440)

Use

Tags

PelB export sequence and Twin-Strep-tag

Expression

Bacterial

Mutation

Promoter

lacIq/Ptrc

Availability

Academic Institutions and Nonprofits only

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pFA6a-12Pk-kanMX6

Plasmid

#49176

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

12Pk

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pFA6a-12Pk-hphMX6

Plasmid

#49177

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

12Pk

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pFA6a-G9-5FLAG-kanMX6

Plasmid

#49181

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

G9-5FLAG

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pFA6a-G9-5FLAG-hphMX6

Plasmid

#49185

Purpose

genomic G9-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

G9-5FLAG

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pFA6a-G11-5FLAG-kanMX6

Plasmid

#49182

Purpose

genomic G11-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Use

Tags

G11-5FLAG

Expression

Yeast

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-T7-HIS3MX6

Plasmid

#20759

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-6xGLY-T7-kanMX6

Plasmid

#20760

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-6xGLY-V5-HIS3MX6

Plasmid

#20777

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge