-
Plasmid#158429PurposeBRET biosensor for detection of free Gβγ in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK3ct
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-NS1-YN
Plasmid#74723PurposeExpress C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsHA and YFP N-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-NS1-YC
Plasmid#74724PurposeExpress C-terminal YC fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsFLAG and YFP C-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-B2-YN
Plasmid#74721PurposeExpress C-terminal YN fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsHA and YFP N-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdRBFC-B2-YC
Plasmid#74722PurposeExpress C-terminal YC fused flock house virus (FHV) B2 protein (2741-2956 nt) for dsRNA labelingDepositorInsertInfluenza A virus NS1 protein, partial
UseDouble-stranded rna labelingTagsFLAG and YFP C-terminal half domainExpressionPlantMutationPromoterCaMV 35SAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGFP FAT
Plasmid#50517PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertprotein tyrosine kinase, domain
UseTagsGFPExpressionMammalianMutationtruncated (NT2630-3268)PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:AVRa1-V1
Plasmid#132357PurposeExpression of the Blumeria graminis f. sp. hordei effector AVRa1-V1 ∆SP from the Zea mays ubiquitin promotorDepositorInsertAVRa1-V1 ∆SP with stop codon
UseTagsExpressionPlantMutationPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGFP FRNK
Plasmid#50518PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertprotein tyrosine kinase, mutated
UseTagsGFPExpressionMammalianMutationtruncated (NT2186-3268)PromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:AVRa1
Plasmid#132356PurposeExpression of the Blumeria graminis f. sp. hordei effector AVRa1 ∆SP from the Zea mays ubiquitin promotorDepositorInsertAVRa1 ∆SP with stop codon
UseTagsExpressionPlantMutationPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSin wPGK-Cre
Plasmid#101242Purposelow-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expressionDepositorInsertCre recombinase
UseLentiviralTagsExpressionMutationsee attached filePromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PTEN
Plasmid#50519PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertPTEN (PTEN Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαq*-BERKY1
Plasmid#158430PurposeBRET biosensor for detection of Gαq-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-GRK2(RH)
UseLentiviralTagsExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PTEN C124A
Plasmid#50520PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertPTEN (PTEN Human)
UseTagsGFPExpressionMammalianMutationC124APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
TN-XXL pRSETB
Plasmid#45796PurposeFluorescent reporter for calcium signalingDepositorInsertTN-XXL
UseTags6xHIS, ECFP, and YFPExpressionBacterialMutationPromoterT7Available sinceJan. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-Venus-173-N
Plasmid#87616PurposeFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-Venus173-N
UseTags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSyn-Gαi*-BERKY1
Plasmid#158427PurposeBRET biosensor for detection of Gαi-GTP in lentiviral vector pLenti-hSynDepositorInsertLyn11-Nluc-ER/K linker-YFP-KB1753-myc
UseLentiviralTagsmycExpressionMammalianMutationPromoterhSynapsinAvailable sinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
GTH-y
Plasmid#81105PurposeGene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…PromoterAvailable sinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PTEN D92A
Plasmid#50521PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertPTEN (PTEN Human)
UseTagsGFPExpressionMammalianMutationD92APromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-DKAR-Nuc
Plasmid#45499DepositorInsertDKAR-Nuc
UseTagsCFP, NLS, and YFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
CFP-Rpp29-C1
Plasmid#134546PurposeExpresses CFP-Rpp29 fusion proteinDepositorInsertRpp29 (POP4 Human)
UseTagsCFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only