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Py2087ACR1

Plasmid

#153032

Purpose

Anion-conducting channelrhodopsin of viral origin. Codon-optimized for mammalian expression (human/mouse).

Depositor

Peter Hegemann

Article

Rozenberg et al Curr Biol. 2020 Dec 21;30(24):4910-4920.

Insert

Py2087ACR1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV (+ enhancer)

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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ATF4 8: (SL-)5’ATF4.GFP

Plasmid

#21855

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-831-1281

Plasmid

#40962

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Use

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 1-830 and 1282-1792

Promoter

Available since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
Septin 11 isoform II-EGFP

Plasmid

#120316

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the C-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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ATF4 9: (SL+)5’ATF4.GFP

Plasmid

#21856

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

ATF4 (Atf4 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Stem loop introduced at the 5' end of the mR…

Promoter

Available since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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eGFP-pk-N273K

Plasmid

#115617

Purpose

Expresses human Parkin N273K in mammalian cells

Depositor

Edward Fon

Article

Tang et al Nat Commun. 2017 Mar 9;8:14697. doi: 10.

Insert

Parkin (PRKN Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

N273K

Promoter

CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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vsv-Sgo1 N61I-GFP

Plasmid

#108497

Purpose

expression of vsv-Sgo1 N61I-EGFP (no PP2A binding)

Depositor

Susanne Lens

Article

Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

Insert

Shugoshin 1 (SGO1 Human)

Use

Tags

EGFP and VSV

Expression

Mammalian

Mutation

no PP2A binding N61I

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6m

Plasmid

#67557

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6m

Use

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-hPMCA4b delta6

Plasmid

#47590

Depositor

Emanuel Strehler

Article

Padanyi et al Biochim Biophys Acta. 2009 Jun;1793(6):1

Insert

PMCA4bdelta6 (ATP2B4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available since

Aug. 26, 2013

Availability

Academic Institutions and Nonprofits only
sspa-GCaMP6m

Plasmid

#67560

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6m

Use

Tags

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
p150-CC1-tagBFP

Plasmid

#222314

Purpose

Inactivates dynein function.

Depositor

Franck Perez

Article

Fourriere et al J Cell Sci. 2016 Sep 1;129(17):3238-50.

Insert

Coiled-coil domain of p150glued (DCTN1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pAN056

Plasmid

#220052

Purpose

Reporter plasmid that expresses a a firefly luciferase gene fused to exons 22-27 of the CFTR gene and a synthetic intron (positive control).

Depositor

Blake Wiedenheft

Article

Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

Insert

fLuc-CFTR (exons 22-27) (CFTR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1318

Plasmid

#217960

Purpose

Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H4 (H4C1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

L63A, F66A, I71A

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCL1317

Plasmid

#217959

Purpose

Expression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.

Depositor

Andrew Bowman

Article

Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755

Insert

Histone H4 (H4C1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

GGG insertion between V65 and F66

Promoter

CMV

Available since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-FRB-Rab5

Plasmid

#214272

Purpose

Mammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-FRB-Rab5A (RAB5A Canis lupus familiaris (Dog))

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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M_NES 54kDa

Plasmid

#201375

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

MAPK_NES-EGFP-4PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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Vd-ARATR-GFP

Plasmid

#208903

Purpose

encodes the arachnotocin receptor (oxytocin/vasopressin-like) from the mite Varroa destructor, tagged with GFP

Depositor

Christian Gruber

Article

Duerrauer et al Sci Rep. 2019 Dec 17;9(1):19295. doi: 10

Insert

ARATR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-4 (b)

Plasmid

#204435

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

I735A

Promoter

CMV

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (a)

Plasmid

#204428

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

R627A K663A R683A

Promoter

CMV

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Interface-1 (c)

Plasmid

#204430

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q659A

Promoter

CMV

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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