EGFP-hPMCA4b delta6
(Plasmid #47590)

• Depositing Lab: Emanuel Strehler
• Publication: Padanyi et al Biochim Biophys Acta. 2009 Jun;1793(6):1023-32. doi: 10.1016/j.bbamcr.2008.11.007. Epub 2008 Nov 27.

Backbone

• Vector backbone: pEGFP-C3
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PMCA4bdelta6
• Alt name: ATPase, Ca++ transporting, plasma membrane 4
• Alt name: ATP2B4
• Species: H. sapiens (human)
• Mutation: 6 C-terminal AA deleted (needed for PDZ binding)
• Entrez Gene: ATP2B4 (a.k.a. ATP2B2, MXRA1, PMCA4, PMCA4b, PMCA4x)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: SV40pA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

x/b splice variant of human PMCA4