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pCMV5-Flag-XBP1s
Plasmid#63680PurposeMammalian expression of Flag tagged XBP1sDepositorInsertXBP1 (XBP1 Human)
UseTagsFlagExpressionMammalianMutationsplicedPromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BAF_L58R
Plasmid#101776PurposeExpresses EGFP tagged mutant BAF (L58R) in human cellsDepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationL58R mutationPromoterEF1aAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
H14_MBP_SUMO-Cys-linker-BAF
Plasmid#101777PurposeExpresses His-MBP-tagged BAF in E.coli (SUMO-cleavable)DepositorInsertBAF (BANF1) (BANF1 Human)
UseTagsHis14-MBP-SUMOExpressionBacterialMutationPromoterT5Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
MultiMate 5 colors
Plasmid#206266PurposeExpression of 5 fluorescently labelled proteins in mammalian cells. Can be used as a CRE-donor (MultiBac system)DepositorInsertH2B (H. Sapiens), Actin and Tubulin (B. taurus), mito mCherry (Synthetic), CyOFP1 (E. quadricolor)
UseRecombinant baculovirus production, cre acceptor…TagsExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFR D837A
Plasmid#11014PurposeRetroviral construct for expressing human EGFR with D837A mutation in human cellsDepositorInsertEGFR D837A (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationD837APromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
EGFR G719S
Plasmid#11013PurposeRetroviral construct for expressing human EGFR with G719S mutation in human cellsDepositorInsertEGFR G719S (EGFR Human)
UseRetroviralTagsExpressionMammalianMutationG719SPromoterAvailable sinceDec. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-Flag-XBP1u
Plasmid#63679PurposeAdenoviral shuttle vector for XBP1u expressionDepositorInsertXBP1 (XBP1 Human)
UseAdenoviralTagsFlagExpressionMammalianMutationunsplicedPromoterCMVAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1_HBB_N
Plasmid#87847PurposepCR2.1-based plasmid holding a 309-bp fragment containing the exon-1-exon-2 splice border of normal human β-globin mRNADepositorInsertHomo sapiens hemoglobin subunit beta (HBB), Normal cDNA fragment (Exon1/2) (HBB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_Smc4-mAID-HaloTAG
Plasmid#217658PurposeRepair template to endogenously tag SMC4 with mAID-Halo TagDepositorInsertSMC4-mAID-HaloTag (SMC4 Human)
UseTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBABE-SSeCKs T751A
Plasmid#107142Purposefull-length murine SSeCKs/AKAP12/Gravin T751A (analogous to T766A mutation in human) in retroviral vectorDepositorInsertSSeCKs T751A (Akap12 Mouse)
UseRetroviralTagsExpressionMammalianMutationchanged threonine 751 to alaninePromotergagAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (OTU+AnkUBD, aa 245-697)
Plasmid#61590PurposeExpresses human TRABID (OTU+AnkUBD domains) in E. coli.DepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-244 and aa 698-708.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
ATG-1929
Plasmid#108712PurposeCBR2opt in pF4Ag expression vector with T7 and CMV promoterDepositorInsertCBR2opt
UseTagsExpressionBacterial and MammalianMutationPromega used directed evolution to create CBR (Cl…PromoterT7, CMVAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP261
Plasmid#104166PurposeBacterial expression plasmid of anti-GFP nanobody with 1x Cysteine for maleimide labelingDepositorInsertAnti-GFP nanobody Enhancer PDB 3K1K (1x Cysteine)
UseTags14x Histidine tag and NEDD8 from Brachypodium dis…ExpressionBacterialMutation1x Cysteine located at bps 493-495 in full sequen…PromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-eIF4E
Plasmid#33252DepositorInserteIF4E (EIF4E Human)
UseRetroviralTagsuntaggedExpressionMammalianMutationunmodifiedPromoterSV40Available sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMMDS DEST
Plasmid#206264PurposeDEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.DepositorTypeEmpty backboneUseCre-donor, multimate/gateway dest vector (multiba…TagsExpressionMutationPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro G3BP1-sgRNA
Plasmid#196197PurposeCas9 + gRNA expression to edit G3BP1 locusDepositorInsertG3BP1 (G3BP1 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-HA-PIK3CA(H1047R;I848V)
Plasmid#33253DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and isoleucine…PromoterSV40Available sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-HA-PIK3CA(H1047R;G320E)
Plasmid#33254DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and glycine 32…PromoterSV40Available sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only
