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Plasmid#224551PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsHA-Twin-StrepExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAAV_hDlx_DIO_ChR2_mCherry
Plasmid#224459PurposeAn AAV vector expressing ChR2-mCherry that is dependent on both Cre and the hDlx enhancerDepositorInsertChR2-mCherry
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBK1290-AAV-dSaCas9
Plasmid#223139PurposeAAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA ScaffoldDepositorTypeEmpty backboneUseAAV and CRISPRTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-RB1
Plasmid#222550PurposePiggyBac transposon plasmid for doxycycline inducible expression of RB1DepositorInsertRB1 (RB1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-KDM5B
Plasmid#222555PurposePiggyBac transposon plasmid for doxycycline inducible expression of KDM5BDepositorInsertKDM5B (KDM5B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
LAMTOR1-APEX2
Plasmid#215556PurposeStable, inducible expression in lysosomeDepositorInsertLAMTOR1-EGFP-Flag tag-APEX2-NES
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
Plasmid#222987PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertCL7_2xNLS_iGeoCas9(C1)_2xNLS_His
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-PH-mCherry-human p53 1-50 (HK180)
Plasmid#206458Purposep53 1-50 expression in Schneider cells for imagingDepositorInsertp53 1-50 (TP53 Human)
UseTagsPH mcherryExpressionInsectMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC8-pA
Plasmid#220760PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC8
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-oScarlet-WPRE-BC6-pA
Plasmid#220770PurposeExpression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertoScarlert-WPRE-BC6
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC6-pA
Plasmid#220758PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC6
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-oScarlet-WPRE-BC11-pA
Plasmid#220775PurposeExpression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertoScarlert-WPRE-BC11
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC2-pA
Plasmid#220754PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC2
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC10-pA
Plasmid#220762PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC10
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC9-pA
Plasmid#220761PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC9
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
GFP-FKBP-LBR
Plasmid#222427PurposeTo express LBR with N-terminal GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLBR (LBR Human)
UseTagsGFP-FKBPExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
LBR(R583Q)-FKBP-GFP
Plasmid#222423PurposeTo express a cholesterol synthesis point mutant of LBR (R583Q) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLBR(R583Q) (LBR Human)
UseTagsFKBP-GFPExpressionMammalianMutationR583QPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
LBR(N547D)-FKBP-GFP
Plasmid#222422PurposeTo express a cholesterol synthesis point mutant of LBR (N547D) with GFP and a FKBP tag for rapamycin-induced heterodimerisation in mammalian cellsDepositorInsertLBR(N547D) (LBR Human)
UseTagsFKBP-GFPExpressionMammalianMutationN547DPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-E696K-EGFP
Plasmid#221554PurposeExpresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationChanged glutamate 696 to lysinePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-oScarlet-WPRE-BC12-pA
Plasmid#220776PurposeExpression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertoScarlert-WPRE-BC12
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only