Showing: 2221 - 2240 of 145410 results
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Plasmid#128584PurposeFor mammalian cell expression of Q61L mutant murine RAC1 carrying a modified hormone-binding domain of murine ERalpha at the N-terminus. Activation of expressed latent fusion protein induced by 4-OHTDepositorInsertRac1 (Rac1 Mouse)
UseRetroviralTagsModified hormone binding domain of ER alphaExpressionMammalianMutationChanged Glutamine61 to Leucine in the RAC1 moiety.PromoterAvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128587PurposeAAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterEF1α (1.1 kb short version)AvailabilityAcademic Institutions and Nonprofits only -
AAV-EF1α1.1-FLPX-rc [ChrimsonR-GFP]
Plasmid#128588PurposeAAV-mediated expression of ChrimsonR-GFP under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner.DepositorInsertChrimsonR-GFP
UseAAVTagsGFPExpressionMammalianMutationChrimson K176R mutantPromoterEF1α (1.1 kb short version)AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-FLPX-rc [ChrimsonR-tdTomato]
Plasmid#128589PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.DepositorHas ServiceAAV8InsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterSynAvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S2
Plasmid#128590PurposeExpresses tagless S2 ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S2 (rpsB E.coli)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S3
Plasmid#128591PurposeExpresses tagless S3 ribosomal protein in E.coliDepositorInsertS3 E.coli ribosomal protein (rpsC E.coli)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S7-S83C/Δ157-178
Plasmid#128592PurposeExpresses tagless S7-S83C, Δ157-178 mutant 30S ribosomal protein in E.coliDepositorInsertS7 E.coli ribosomal protein (rpsG E.coli)
UseTagsExpressionBacterialMutationS83C, Δ157-178PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S10
Plasmid#128594PurposeExpresses tagless S10 ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S10 (rpsJ E.coli)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S14
Plasmid#128596PurposeExpresses tagless 30S ribosomal subunit protein S14 in E.coliDepositorInsert30S ribosomal subunit protein S14 (rpsN E.coli)
UseTagsExpressionBacterialMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pRSF-1b-S19-T48C
Plasmid#128597PurposeExpresses tagless S19-T48C mutant 30S ribosomal protein in E.coliDepositorInsert30S ribosomal subunit protein S19 (rpsS E.coli)
UseTagsExpressionBacterialMutationT48CPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pIIS18-SapI
Plasmid#128599PurposeEntry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymesDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-GFP (MoonTag-Nb-GFP)
Plasmid#128602PurposeExpresses nanobody-2H10 fused to super folded GFP (sfGFP) in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and sfGFPExpressionMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
Nb-gp41-Halo (MoonTag-Nb-Halo)
Plasmid#128603PurposeExpresses nanobody-2H10 fused to Halo in mammalian cells. It recognizes gp41 peptide array (MoonTag peptide array).DepositorInsertNanobody_gp41
UseLentiviralTagsGB1 and HaloExpressionMutationPromoterSFFVAvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-24xPP7 (MoonTag translation reporter)
Plasmid#128604PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailabilityAcademic Institutions and Nonprofits only -
24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)
Plasmid#128605PurposeExpresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailabilityAcademic Institutions and Nonprofits only -
12xMoonTag-12xSunTag-kif18b-24xPP7
Plasmid#128606PurposeExpresses 12xgp41 peptide array (MoonTag peptide array), followed by 12xgcn4 peptide array (SunTag peptide array), kif18b gene and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertsUseTagsExpressionMammalianMutationPromoterPcmvAvailabilityAcademic Institutions and Nonprofits only -
MStart-36xMashTag-BFP-3xStop-24xPP7 (Moonstart-MashTag reporter)
Plasmid#128607PurposeExpresses 36xMashTag with MoonTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsert36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailabilityAcademic Institutions and Nonprofits only -
SStart-36xMashTag-BFP-3xStop-24xPP7 (Sunstart-MashTag reporter)
Plasmid#128608PurposeExpresses 36xMashTag with SunTag frame as mainframe, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsert36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailabilityAcademic Institutions and Nonprofits only -
uORF1-Mreinit- 36xMashTag-BFP-3xStop-24xPP7 (uORF-MashTag reporter)
Plasmid#128609PurposeExpresses uORF1 -36xMashTag with MoonTag as readout for reinitiation, followed by BFP linker and 24 repeats of PP7 hairpins in 3’ UTR.DepositorInsertuORF1-36xMashTag
UseTagsExpressionMammalianMutationPromoterPcmvAvailabilityAcademic Institutions and Nonprofits only -
Multi-frame tag
Plasmid#128610PurposeIn the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.DepositorInsertMF tag
UseTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 2221 - 2240 of 145410 results