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pRSFDuetG-DmDCP1_328-366 (MCSII)-mut1_G

Plasmid

#146403

Purpose

Bacterial Expression of DmDCP1_328-366-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366-mut1 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_328-366 (MCSII)-mut2_G

Plasmid

#146404

Purpose

Bacterial Expression of DmDCP1_328-366-mut2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366-mut2 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_328-366 (MCSII)-mut3_G

Plasmid

#146405

Purpose

Bacterial Expression of DmDCP1_328-366-mut3

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366-mut3 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_328-366 (MCSII)-WT_G

Plasmid

#146406

Purpose

Bacterial Expression of DmDCP1_328-366

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_328-366 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-DmDCP2-V5His6_H

Plasmid

#146436

Purpose

Insect Expression of DmDCP2

Depositor

Elisa Izaurralde

Insert

DmDCP2 (DCP2 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1_327-372_H

Plasmid

#146500

Purpose

Insect Expression of DmDCP1_327-372

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_327-372 (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_1-127 (MCSII)_E

Plasmid

#146173

Purpose

Bacterial Expression of DmDCP1_1-127

Depositor

Elisa Izaurralde

Insert

DmDCP1_1-127 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_Nbr

Plasmid

#186662

Purpose

Donor plasmid to endogenously tag Nbr gene in Drosophila melanogaster at the C-terminal.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Nbr C-terminal 3xFlag-3xHA Tag Donor Plasmid (Nbr Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA2

Plasmid

#186664

Purpose

Nbr C-tag sgRNA2 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 2 Plasmid (Nbr Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA1

Plasmid

#186663

Purpose

Nbr C-tag sgRNA1 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 1 Plasmid (Nbr Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Shu_sgRNA

Plasmid

#186656

Purpose

shu sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Shu sgRNA Plasmid (shu Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_nonPuro_OSS

Plasmid

#186652

Purpose

Cas9 plasmid to express the Cas9 in OSS cells

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Cas9 Expression Plasmid

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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puc19_N_tag

Plasmid

#186650

Purpose

Empty donor plasmid.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

N-terminal 3xFlag-3xHA Tag

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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PUC19_C_tag

Plasmid

#186659

Purpose

Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

C-terminal 3xFlag-3xHA Tag

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor_N_FH_GLE

Plasmid

#186657

Purpose

Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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VIR_sgRNA

Plasmid

#186661

Purpose

VIR sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

VIR sgRNA Plasmid (vir Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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I282L

Plasmid

#184308

Purpose

To study dynamics of G-actin using NMR spectroscopy.

Depositor

Michael Rosen

Article

Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

Insert

Act5C (Act5C Fly)

Use

Tags

B-Thymosin followedby hexahistidine tag

Expression

Yeast

Mutation

I282L

Promoter

AOX1

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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I175L

Plasmid

#184322

Purpose

To study dynamics of G-actin using NMR spectroscopy.

Depositor

Michael Rosen

Article

Ali et al Nat Struct Mol Biol. 2022 Apr;29(4):320-

Insert

Act5C (Act5C Fly)

Use

Tags

Hexahistidine followed by B-thymosin

Expression

Yeast

Mutation

I175L

Promoter

AOX1

Available since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pTL370

Plasmid

#69880

Purpose

attB flanked genomic Cirl for integration at attP sites.

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTL464

Plasmid

#69881

Purpose

a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

Depositor

Robert Kittel, Tobias Langenhan

Article

Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

Insert

CIRL (Cirl Fly)

Use

Phic31-integration vector

Tags

Expression

Mutation

Promoter

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge