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Plasmid#185705PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PDCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(PDCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(SCA)-6xHis-NLS(SV40)
Plasmid#185703PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(SCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(SCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(PCA)-6xHis-NLS(SV40)
Plasmid#185704PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(PCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(PCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-NLS(SV40)-anCas(BCA)-6xHis-NLS(SV40)
Plasmid#185702PurposeCMV and T7 promoter expression plasmid for human codon usage anCas(BCA) with an N-terminal NLS and C-terminal NLSDepositorInsertanCas(BCA)
UseTags6xHis-NLS and NLSExpressionMammalianMutationoptimized for human codon usagePromoterCMV and T7Available sinceJune 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGK-Ptrf-mKate2
Plasmid#128765PurposeFluorescent mKate reporter for PtrfDepositorInsertPtrf promoter (Cavin1 Mouse)
UseTagsExpressionMammalianMutationPromoterPtrfAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGK-Ldb3-mKate2
Plasmid#128766PurposeFluorescent mKate reporter for Ldb3DepositorInsertLdb3 promoter (Ldb3 Mouse)
UseTagsExpressionMammalianMutationPromoterLdb3Available sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S12TAG
Plasmid#107155PurposeMBP-rPKL construct for production and purification of rat PKL pS12DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 12 to TAG for phosphoserine insertionPromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
MBP-rPKL S113TAG
Plasmid#107157PurposeMBP-rPKL S113TAG for production and purification of rat PKL pS113DepositorInsertMBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)
UseTagsC-term 6-his and N-term MBPExpressionBacterialMutationSerine 113 changed to TAG for phosphoserine inser…PromoterPBADAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAav-IQGAP1-PQS1-3xFLAG
Plasmid#84885PurposerAAV-based template for genome engineering of the IQGAP1 C-terminus containing PQS1 and 3xFLAG tags and a selection cassetteDepositorInsertsUseAAVTagsPQS1 3xFLAGExpressionMutationPromoternoAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAav-TP53-PQS1-3xFLAG
Plasmid#84884PurposerAAV-based template for genome engineering of the p53 C-terminus containing PQS1 and 3xFLAG tags and a selection cassetteDepositorInsertsUseAAVTagsPQS1 3xFLAGExpressionMutationPromoterno and noAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS1499
Plasmid#29247PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle28 (CCL27 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceNov. 7, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1498
Plasmid#29246PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle27 (CCL27 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceNov. 7, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1493
Plasmid#29241PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle22 (CCKBR Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceNov. 7, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1568
Plasmid#29265PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle97 (GPX3 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceOct. 21, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1500
Plasmid#29248PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).DepositorInsertPle29 (CCL27 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsIntron-LacZ-NLSExpressionMutationPromoterAvailable sinceOct. 20, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS1215
Plasmid#29109PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle97 (GPX3 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceOct. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCag SE (Self-excising) FlpO-2A-Cre EV
Plasmid#130986Purposeepisomal expression of FlpO and Cre recombinases, self excisingDepositorInsertFlpO-2A-Cre
UseCre/Lox and Unspecified; Episomal expression vect…TagsExpressionMammalianMutationprotamine intron added to FlpO to prevent bacteri…PromoterCMV/Chick β-actin (CAG)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG-CSF2RB
Plasmid#125750PurposeLentiviral Expression Vektor with CSF2RB wildtype cDNA and IRES eGFPDepositorInsertCSF2RB(wt) (CSF2RB 2694, Human)
UseLentiviralTagsExpressionMutationcodon optimizedPromoterSFFVAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2
Plasmid#112669PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.DepositorInserttdT
UseAAV; Donor plasmid for recombinase-mediated casse…TagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-nSpRY(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES636)
Plasmid#185491PurposeCMV and T7 promoter expression plasmid for human codon usage nSpRY(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFPDepositorInserthuman codon usage nSpRY(D10A)-BPNLS-P2A-eGFP
UseIn vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-eGFPExpressionMammalianMutationD10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only