pEMS1499
(Plasmid
#29247)
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PurposePleiades (Ple) MiniPromoter expression pattern described in de Leeuw et al., MTM, 2014 (PMID: 24761428).
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29247 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepEMS1313
- Backbone size w/o insert (bp) 18843
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Vector typePleiades Promoter Project [sic, Pleaides Plieades]
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePle28
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Alt nameCCL27
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Alt nameSmall inducible cytokine A27 precursor
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SpeciesH. sapiens (human)
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Insert Size (bp)2414
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Entrez GeneCCL27 (a.k.a. ALP, CTACK, CTAK, ESKINE, ILC, PESKY, SCYA27)
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Tag
/ Fusion Protein
- Intron-LacZ-NLS (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site FseI (unknown if destroyed)
- 3′ cloning site AscI (unknown if destroyed)
- 5′ sequencing primer Unknown (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEMS1499 was a gift from Elizabeth Simpson (Addgene plasmid # 29247 ; http://n2t.net/addgene:29247 ; RRID:Addgene_29247) -
For your References section:
Targeted CNS Delivery Using Human MiniPromoters and Demonstrated Compatibility with Adeno-Associated Viral Vectors. de Leeuw CN, Dyka FM, Boye SL, Laprise S, Zhou M, Chou AY, Borretta L, McInerny SC, Banks KG, Portales-Casamar E, Swanson MI, D'Souza CA, Boye SE, Jones SJ, Holt RA, Goldowitz D, Hauswirth WW, Wasserman WW, Simpson EM. Mol Ther Methods Clin Dev. 2014 Jan 8;1:5. 10.1038/mtm.2013.5 PubMed 24761428