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pEGFP-HA3-STONIN1

Plasmid

#177580

Purpose

Expresses Stonin1 in mammalian cells

Depositor

Juan Bonifacino

Article

Martina et al J Cell Biol. 2001 May 28;153(5):1111-20.

Insert

STONIN1

Use

Tags

3XHA and GFP

Expression

Mammalian

Mutation

V334A- please see depositor comments

Promoter

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXL2-WT

Plasmid

#194584

Purpose

Mammalian Expression of mEGFP-FOXL2 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXL2 (FOXL2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-Sec61β

Plasmid

#174466

Purpose

Express EGFP with endoplasmic reticulum-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with endoplasmic reticulum-targeting sequence

Use

Tags

Sec61 subunit beta

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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RCASBP-Y NHA-EGFP

Plasmid

#11481

Depositor

William Pavan

Article

Loftus et al DNA Res. 2001 Oct 31. 8(5):221-6.

Insert

Enhanced Green Fluoresecent Protein

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 22, 2006

Availability

Academic Institutions and Nonprofits only
pRRL.SP107.gp47.eGFP

Plasmid

#30472

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 May 5. ():.

Insert

SP107/gp47/eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsHelz_AG

Plasmid

#148799

Purpose

Mammalian Expression of HsHelz

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

HsHelz (HELZ Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-Bad-4E-pEGFP-C1

Plasmid

#166740

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, Bad with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

Bad-4E (BAD Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

4 hydrophobic residues in the BH3 region mutated …

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMS-eGFP-RTP801

Plasmid

#65057

Purpose

Expression of RTP801 in mammalian cells

Depositor

Cristina Malagelada, Lloyd Greene

Article

Malagelada et al J Neurosci. 2006 Sep 27;26(39):9996-1000

Insert

DDIT4 (Ddit4 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
6x Halo-EGFP- 2x PACT

Plasmid

#107265

Purpose

6x Halo-EGFP- 2x PACT construct downstream of T7 promotor for in vitro transcription

Depositor

Michael Lampson

Article

Akera et al Science. 2017 Nov 3;358(6363):668-672. d

Insert

6x Halo-EGFP- 2x PACT

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

T7

Available since

July 4, 2018

Availability

Academic Institutions and Nonprofits only

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eMagAF-EGFP-Mito

Plasmid

#162243

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Article

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Insert

eMagAF-EGFP-OMP25

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-INPP5E-CAAX

Plasmid

#202746

Purpose

express plasma membrane targeted fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pMA-MsgRNA-EGFP

Plasmid

#80794

Purpose

For insertion of gRNA array containing 11-30 gRNA modules

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-pegRNA_KCNA1-EGFP

Plasmid

#159787

Purpose

S. pyogenes pegRNA for C to A mutation at the KCNA1 site of human cells using prime editing

Depositor

Xiaolong Wang

Article

Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

Insert

spacer of pegRNA targeting KCNA1 gene (KCNA1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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HT116_pAAV_hSyn-DiO-SomQuasAr6b_EGFP

Plasmid

#190879

Purpose

Cre-on expression of soma-targeted QuasAr6b under an neuronal promoter

Depositor

Adam Cohen

Article

Tian et al Nat Methods. 2023 Jul;20(7):1082-1094. d

Insert

soma-targeted QuasAr6b

Use

AAV

Tags

EGFP

Expression

Mutation

Promoter

hSyn

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL-pEGFP-C1

Plasmid

#166735

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, BimEL

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL (Bcl2l11 Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-7xTRE-3xeGFP

Plasmid

#191206

Purpose

GFP expression under the control of TRE promotor

Depositor

Janelia Viral Tools

Article

Glaser et al Nat Methods. 2022 May;19(5):613-619. doi

Insert

3X GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EGFP-C3-Cep123

Plasmid

#73330

Purpose

Lentiviral vector encoding Cep123 isoform 1 with an N-terminal EGFP tag

Depositor

Manuel Thery

Insert

Cep123 (CEP89 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2(plus)EGFP_H

Plasmid

#146446

Purpose

Bacterial Expression vector

Depositor

Elisa Izaurralde

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR - EGFP sgRNA 3

Plasmid

#51762

Purpose

Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

Depositor

Feng Zhang

Article

Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

Inserts

Cas9
Puromycin resistance
EGFP sgRNA 3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pQCXIP-Rx3(A7)-mEGFP

Plasmid

#35531

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Rx3(A7)-mEGFP

Use

Retroviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

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