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pEPQD1CB0905

Plasmid

#177068

Purpose

MoClo Level 1, position 1, transcriptional unit for transient expression of gal4AD-phiC31 driven by 35S promoter

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

gal4AD-phiC31

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPKK0CM0480

Plasmid

#177037

Purpose

Level 0 promoter binding site part for MoClo assembly, nonstandard overlaps, CCAT-TACT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

2x opattB binding site plus minimal CaMV 35S promoter plus partial TMV 'UTR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0705

Plasmid

#177035

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

phiC31 integrase plus SV40 nuclear localization sequence (PKKKRKV)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0812

Plasmid

#177034

Purpose

Level 0 N-terminal tag part for MoClo assembly, CCAT-AATG

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

gal4 activation domain plus GGSGGGGSGG linker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0910

Plasmid

#177033

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

loganic acid O-methyltransferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0766

Plasmid

#177032

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

strictosidine synthase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0764

Plasmid

#177030

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

secologanin synthase; CYP72C

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0763

Plasmid

#177029

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

loganic acid O-methyltransferase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0762

Plasmid

#177028

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganic acid hydroxylase; CYP72A224

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0062

Plasmid

#177027

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

7-deoxyloganetic acid glucosyl transferase; UGT709C2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0060

Plasmid

#177024

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

iridoid synthase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0059

Plasmid

#177023

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

8-hydroxygeraniol oxidoreductase; 10-hydroxygeraniol oxidoreductase; alcohol dehydrogenase 10

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0063

Plasmid

#177021

Purpose

Level 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

geraniol synthase

Use

Synthetic Biology

Tags

Expression

Mutation

transit peptide removed

Promoter

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hNKD2

Plasmid

#174329

Purpose

encodes human NKD2 cDNA with a C-terminal MycHis Tag.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Use

Tags

MycHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJo55

Plasmid

#159623

Purpose

An empty HIS3 plasmid for translation factor eIF4E complementation test in yeast, strain Jo55. Golden Gate cloning using BsaI. Selection with RFP after cloning.

Depositor

Vasiliy Taranov

Article

Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJo55-CA1

Plasmid

#159624

Purpose

Plasmid pJo55, which contains positive control (C. annuum eIF4E-1) for complementation test in Jo55 yeasts.

Depositor

Vasiliy Taranov

Article

Lebedeva et al Biochemistry (Mosc). 2021 Sep;86(9):1128

Insert

C. annuum eIF4E-1

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD-MtrxRFP2

Plasmid

#172323

Purpose

fluorescent biosensor for the redox of mitochondrial specific thioredoxin (Trx2)

Depositor

Huiwang Ai

Article

Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

Insert

human thioredoxin 2, fused with a redox-sensitive RFP through Gly-Ser-rich linker

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pJCC_084 Cas12i1 D647A

Plasmid

#171671

Purpose

For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1 D647A

Use

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 647 to alanine

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 S389,398 A

Plasmid

#129485

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 S389,398 A (GLO3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Mutation of serines 389 and 398 to alanine

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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p415 Glo3 S389,398 D

Plasmid

#129486

Purpose

Expression in S. cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3 S389,398 D (GLO3 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Mutation of serines 389 and 398 to Asp

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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