Addgene

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RnBarr2-mTurquoise2

Plasmid

#137792

Purpose

Visualization of beta-arrestin2

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin2 (Arrb2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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RnBarr1-mTurquoise2

Plasmid

#137789

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta4

Plasmid

#101292

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

76-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRB

Plasmid

#193568

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Use

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP-VP16

Plasmid

#183923

Purpose

VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-VP16

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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PCDHGC5-EGFP

Plasmid

#119933

Purpose

Protocadherin gamma C5 expression (EGFP-tag at the C-terminus).

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta6

Plasmid

#101294

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

375-1354 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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mVenus-RnBarr1

Plasmid

#137796

Purpose

Visualization of beta-arrestin1

Depositor

Dorus Gadella

Article

Pietraszewska-Bogiel et al ACS Omega. 2020 Feb 3;5(6):2648-2659. do

Insert

Rat beta-arrestin 1 (Arrb1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCLC3sGFP

Plasmid

#52423

Purpose

mammalian expression of rat CLC3 short transcript fused to EGFP

Depositor

Steven Weinman

Article

Li et al Am J Physiol Cell Physiol. 2002 Jun;282(

Insert

CLC3 (Clcn3 Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6s

Plasmid

#67556

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6s

Use

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
EGFP-GEM

Plasmid

#111191

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Article

Piddini et al EMBO J. 2001 Aug 1. 20(15):4076-87.

Insert

GEM (GEM Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T2A

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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mNeongreen-pGBD(PKN1 codon optimized)

Plasmid

#129634

Purpose

Visualization of active RhoA

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

Insert

RhoA binding doamin of PKN1(30 - 100) codon optimized

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVdel

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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delta-EGFP

Plasmid

#119223

Purpose

GABAA receptor expression (delta subunit with EGFP tag at the C-terminus)

Depositor

Angel de Blas

Article

Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

Insert

GABRD (Gabrd Rat)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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CFF-EGFP

Plasmid

#86061

Purpose

A mammalian expression plasmid encoding fibrocystin mimentic clone CFF (CD8a luminal domain, fibrocystin transmembrane and cytosolic domains) with C-terminus EGFP.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Fibrocystin (Pkhd1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

contains luminal and transmembrane domains of CD8…

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Plasmid

#173866

Purpose

Mammalian expression of mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax (opto-Cdc42)

Depositor

Takao Nakata

Article

Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

Insert

mVenus-NES-SspB-ITSN1-DHPH-P2A-iLIDcaax

Use

Tags

C-terminal CAAX-box and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHJ424

Plasmid

#20682

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Ezrin (EZR Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pGP-CMV-NEW-fRGECO2

Plasmid

#125248

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Use

Tags

NES signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK165_Mem-mCherry-4.1G-CTD

Plasmid

#46362

Depositor

Iain Cheeseman

Article

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1G C-terminal domain (EPB41L2 Human)

Use

Tags

Mem (N-terminal 20 amino acids of neuromodulin fo…

Expression

Mammalian

Mutation

C-terminal domain from 886-1005 amino acids

Promoter

CMV

Available since

Aug. 5, 2013

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP4-VPR

Plasmid

#183924

Purpose

VPR activation domain coupled to catalytically inactive dCas9 for localization; contains a GFP tetramer fpr labeling and as a spacer

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP4-VPR

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pOIMars1CyN

Plasmid

#75221

Purpose

Mammalian cell surface expression of fluorogen activating protein Mars1Cy

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

ss-HA-Mars1Cy-CD80(237-306)

Use

Tags

HA epitope

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 31, 2016

Availability

Academic Institutions and Nonprofits only

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