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Plasmid#58871PurposeMESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmcsg7-mCRT(WT)
Plasmid#83505PurposeBacterial expression of CalreticulinDepositorInsertCALR (Calr Mouse)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceOct. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
GAL4-mNFAT1(2-144)
Plasmid#11108PurposeExpresses mouse NFAT1 (amino acids 2-144) fused to GAL4 DNA binding domain in mammalian cellsDepositorInsertNFAT1 (Nfatc2 Mouse)
UseTagsGAL4-DBDExpressionMammalianMutationContains NFAT1 amino acids 2-144; L78P and P140Q …PromoterAvailable sinceApril 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4H
Plasmid#108368PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with MVE xrRNA and 4H probe binding sites (TPI1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:Lucia:bgH
Plasmid#135293PurposeTranscriptional unit for constitutive expression of Lucia luciferase in mammalian cellsDepositorInsertCMV:Lucia:bGH
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-globin_del5UTR_PTC39-xrRNA-4H
Plasmid#108376PurposeFRT integration of beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS G_tTA-BFP
Plasmid#58856PurposeMESA target chain with mCherry ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (4x TBE2 - mut)
Plasmid#16600DepositorInsertc-myc promoter (4xTBE2 mut) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationmutant TBE2 sitesPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCI-globin_del5UTR_PTC39-xrRNA-4H
Plasmid#108367PurposeExpresses beta-globin reporter with premature termination codon (PTC39); enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)DepositorInsertPTC39 beta-globin reporter with MVE xrRNA and 4H probe binding sites
UseTagsExpressionMammalianMutationPTC39PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DARPinOFF7
Plasmid#182397PurposeBacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusionDepositorInsertVNp-DARPinOFF7
UseTagsVesicle Nucleating peptide (VNp)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-tetR-T2A-tSapphire
Plasmid#176312Purposeconstruct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-SapphireDepositorInsertstetR
T-sapphire
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
dTomato_LD 0_CS M_tTA-BFP
Plasmid#58875PurposeMESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRAB18-GFP
Plasmid#106984PurposeABA signaling reporter, GFP under the RAB18 promoterDepositorInsertsGFP
UseTagsExpressionPlantMutationPromoterRAB18Available sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCin4-3xFLAG-MKL1 S449A/T450A/S454A
Plasmid#19845DepositorInsertMKL1 S449A/T450A/S454A (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationchanged Serine 449, Threonine 450 and Serine 454 …PromoterAvailable sinceDec. 16, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-FLAG-Dicer-delPAZ
Plasmid#51367DepositorInsertDicer (DICER1 Human)
UseTagsFLAGExpressionMammalianMutationFrom N terminus to PAZ domain deletionPromoterCMVAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGST2-CeEP55
Plasmid#21501DepositorInsertCEP55-EABR (ESCRT and ALIX Binding Region) domain (CEP55 Human)
UseTagsGSTExpressionBacterialMutationQ230 (CAG changed to TAA), Q218 (CAA changed to T…PromoterAvailable sinceFeb. 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1puro-shKIBRA-A
Plasmid#40888DepositorInsertshKIBRA-A (WWC1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1puro-shKIBRA-B
Plasmid#40889DepositorInsertshKIBRA-B (WWC1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-Myc-SAMTOR
Plasmid#100517PurposeExpresses Myc tagged SAMTOR in mammalian cellsDepositorInsertSAMTOR (BMT2 Human)
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only