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Plasmid#207539PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cellsDepositorInsertHaloTag with internal PuroR cassette flanked by human LC3B locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.4Ch (pc2393)
Plasmid#211730PurposeExpresses MBD2 (aa236-414) mCherry tagged in insect cellsDepositorInsertMBD2.4 aa236-414
UseTagsmCherryExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.3Ch (pc2392)
Plasmid#211729PurposeExpresses MBD2 (aa222-414) mCherry tagged in insect cellsDepositorInsertMBD2.3 aa222-414
UseTagsmCherryExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.5R (pc2395)
Plasmid#211727PurposeExpresses MBD2 (aa153-221) RFP tagged in insect cellsDepositorInsertMBD2.5 aa153-221
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.6R (pc2397)
Plasmid#211725PurposeExpresses MBD2 (aa153-235) RFP tagged in insect cellsDepositorInsertMBD2.6 aa153-235
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MBD2.1mR (pc2391)
Plasmid#211723PurposeExpresses MBD2 (aa1-152) RFP tagged in insect cellsDepositorInsertMBD2.1 aa1-152
UseTagsRFPExpressionInsectMutationPromoterPolyhedrin promotorAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB_MBD2.2-GFP (pc2083)
Plasmid#211722PurposeExpresses MBD2 (aa153-414) GFP tagged in insect cellsDepositorInsertMBD2.2 aa153-414
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB_MBD2.1-GFP (pc2082)
Plasmid#211721PurposeExpresses MBD2 (aa1-152) GFP tagged in insect cellsDepositorInsertMBD2 aa1-152
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2.9Ch (pc2385)
Plasmid#211720PurposeExpresses MeCP2 (aa163-309) mCherry tagged in insect cellsDepositorInsertMeCP2 aa163-309
UseTagsmCherryExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2G.9 (pc1574)
Plasmid#211719PurposeExpresses MeCP2 (aa163-309) GFP tagged in insect cellsDepositorInsertMeCP2 aa163-309
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB-MeCP2G.8 (pc1573)
Plasmid#211718PurposeExpresses MeCP2 (aa310-492) GFP tagged in insect cellsDepositorInsertMeCP2 aa310-492
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB MBD2a-2-strep (pc1794)
Plasmid#211715PurposeExpresses fl. MBD2 2xStrep tagged in insect cellsDepositorInsertMBD2
UseTags2xStrepExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFB MBD2a-C-GFP (pc1565)
Plasmid#211712PurposeExpresses fl. MBD2 GFP tagged in insect cellsDepositorInsertMBD2
UseTagsGFPExpressionInsectMutationPromoterPolyhedrin PromoterAvailable sinceFeb. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-FL
Plasmid#171602PurposeExpresses full length S. pombe Mdn1 in insect cells.DepositorInsertMdn1
UseTagsHis6 tagExpressionInsectMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQadd1F
Plasmid#135660PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQadd1R
Plasmid#135661PurposeClostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.DepositorInsertIntron containing lox site
UseCre/LoxTagsExpressionBacterialMutationPromoterpFerAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFB HTB_hMSH6_T1219D
Plasmid#135466PurposeExpression human MSH6_T1219D mutant protein using baculovirusDepositorInserthuman MSH6_T1219D (MSH6 Human)
UseTagsHis-tagExpressionInsectMutationchanged threonine to aspartic acidPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM55261
Plasmid#153210PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Bar selection cassette and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM55273
Plasmid#153211PurposeLevel 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB m Rosella I
Plasmid#71247Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.DepositorInsertRosella
UseTagsyeast mitochondrial targeting sequence of citrateā¦ExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only