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Plasmid#220595PurposeConstitutive expression of a single-cell discriminating version of AausFP1 fluorescent protein.DepositorInsertAausFP1
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_AAVS1-OsTIR1-F74G-SNAP
Plasmid#217665PurposeRepair template to integrate OsTIR1-F74G-SNAP into the human AAVS1 safe harbour locusDepositorInsertOsTir1 F74G-SNAP-Blast-AAVS1
UseTagsSNAPExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS-HsProm1s1(W795R)-StayGold
Plasmid#211334PurposeVector for stable mammalian gene editing with C-terminally StayGold tagged human Prom1s1 (W795R)DepositorInsertProminin 1 (PROM1 Human)
UseAAVTagsStayGoldExpressionMutationChanged Trp-795 to ArgPromoterEF1aAvailable sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro XLone-CCND2
Plasmid#179845Purposedonor plasmid for inducible expression of CCND2 in human cellsDepositorInsertCCND2 (CCND2 Human)
UseCRISPR, Luciferase, and TALEN ; Donor plasmidTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Blasticidin XLone-eGFP
Plasmid#159754PurposeAAVS1 donor plasmid for targeted inducible eGFP expression in human cellsDepositorInsertall-in-one tet-on system
UseCRISPR and TALEN; Donor plasmid for targeted knoc…TagsExpressionMammalianMutationPromoterTRE3GS inducible promoterAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-UDP1.0-WPRE
Plasmid#220849PurposeAAV virus packaging plasmid for neuronal expression of an extracellular UDP sensor (UDP1.0)DepositorInsertUDP1.0
UseAAVTagsExpressionMutationPromoterhuman Synapsin (hSyn)Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hIsl2-EGFP
Plasmid#153206PurposeHuman Isl2 (hIsl2) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN-eGFP-oPRE
Plasmid#218681PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (SYN) promoter. It also contains an optimised and truncated version of WPRE as a regulatory element.DepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGS96_EGFP(A206K)-I3-01 AAVS donor
Plasmid#211865PurposeI3-01 transgene integration at AAVS1 safe harbor locusDepositorInsertI3-01
UseCRISPRTagsExpressionMutationN/APromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-YTH-EGFP
Plasmid#209322PurposeAAV packaging vector containing a APOBEC1-YTH expression, a P2A-EGFP expression cassette,.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-APOBEC1-YTHmut-EGFP
Plasmid#209323PurposeAAV packaging vector containing a APOBEC1-YTHmut expression cassette, a P2A-EGFP expression cassette.DepositorInsertAPOBEC1--YTHmut-HA
UseAAVTagsHAExpressionMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1-YTH
Plasmid#209319PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTH cassette.DepositorInsertAPOBEC1-YTH-HA
UseAAVTagsHAExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-EGFP-APOBEC1-YTHmut
Plasmid#209320PurposeAAV packaging vector containing a EGFP-P2A expression cassette, APOBEC1-YTHmut cassette.DepositorInsertAPOBEC1-YTHmut-HA
UseAAVTagsHAExpressionMutationYTH domain lacks AA 385-409 comprising the m6A-bi…PromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 LAMP1-mNeonGreen HRD
Plasmid#207549PurposeHomologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cellsDepositorInsertTET inducible LAMP1-mNeonGreen
UseTagsmNeonGreenExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-LC3B HRD
Plasmid#207551PurposeHomologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-LC3B
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 EGFP-GABARAPL1 HRD
Plasmid#207552PurposeHomologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cellsDepositorInsertTET inducible EGFP-GABARAPL1
UseTagsEGFPExpressionMammalianMutationPromoterEndogenousAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-sgCTG-CMV-GFP
Plasmid#216734PurposeAAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.DepositorArticleInsertsgCTG
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6 and CMVAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-SpCas9D10A nickase
Plasmid#216736PurposeExpresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRTagsExpressionMammalianMutationD10APromoterEF-1-alpha coreAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-H2BmTagBFP2-oG
Plasmid#217976PurposeAAV transfer plasmid to express H2B-BFP and codon-optimized Rabies G proteinDepositorInsertsH2BmTagBFP2
oG
UseAAVTagsmTAGBFP2ExpressionMutationPromoterhSynAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-K-SPOTIT(R271F)
Plasmid#191492PurposeGreen fluorescent-based opioid sensor for the kappa opioid receptor with reduced Gai coupling in AAV viral vector under a CAG promoterDepositorInsertK-SPOTIT(R271F)
UseAAVTagsExpressionMutationR271F in ORPromoterCAGAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only