Addgene

We narrowed to 4,143 results for: Pet2;

Showing: 3271 - 3300 of 4143 results

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TEN

Plasmid

#12498

Depositor

David Baker

Article

Dantas et al J Mol Biol. 2003 Sep 12. 332(2):449-60.

Insert

TEN

Use

Tags

6xHis

Expression

Bacterial

Mutation

RosettaDesign was used to design sequences. Align…

Promoter

Available since

March 13, 2007

Availability

Academic Institutions and Nonprofits only
pET-21a(+)-HP RecR

Plasmid

#12620

Depositor

SeWon Suh

Article

Lee et al EMBO J. 2004 May 19. 23(10):2029-38.

Insert

Helicobacter pylori RecR (recR Helicobacter pylori)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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C064

Plasmid

#174353

Purpose

Plasmid for expressing frog PABPC1 (C-His) in E.coli

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (pabpc1.S Frog)

Use

Tags

6xHIS

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-mi3

Plasmid

#159995

Purpose

Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyCatcher003-mi3

Use

Tags

C-Tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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NW9

Plasmid

#133442

Purpose

Covalently circularized nanodisc NW9 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-AlaRS

Plasmid

#224362

Purpose

Express AlaRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al bioRxiv 2024.06.19.599772

Insert

AlaRS (alaS E. coli)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag003-sfGFP

Plasmid

#133454

Purpose

Expresses SpyTag003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-sfGFP

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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RpHLuorin2

Plasmid

#180227

Purpose

for bacterial expression of recombinant ratiometric pHluorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2

Use

Tags

His-tag followed by thrombin cleavage site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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JMC044-B12

Plasmid

#58752

Purpose

bacterial expression of PDB 4NW3

Depositor

Cheryl Arrowsmith

Insert

PDB:4NW3 (KMT2A Human)

Use

Tags

His

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Promoter

T7

Available since

Aug. 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBEL1994

Plasmid

#139898

Purpose

Expression plasmid for LetB PLL2 deletion (without TM region)

Depositor

Gira Bhabha, Damian Ekiert

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta229-239, D228G, L240G)-6xHis

Promoter

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pKS1

Plasmid

#84079

Purpose

Expresses the non-toxic fragment C of tetanus toxin

Depositor

Neil Fairweather

Article

Sinha et al Mol Microbiol. 2000 Sep;37(5):1041-51.

Insert

Tetanus neurotoxin fragment C

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 21, 2018

Availability

Academic Institutions and Nonprofits only

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S48I48 ELP

Plasmid

#68394

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of serine guest residue followed by 48 repeats of isoleucine guest residue

Depositor

Andrew MacKay

Article

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

S48I48 ELP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pC28-LIC-sfGFP-His

Plasmid

#165479

Purpose

pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

Depositor

Renato Bruni

Article

Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

Type

Empty backbone

Use

Tags

TEV-GFP-6xHis

Expression

Bacterial

Mutation

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pBJG1 T7-8xHis-OGT

Plasmid

#154271

Purpose

Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588

Insert

O-GlcNAc Transferase (OGT Human)

Use

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

bacterial codon optimized

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pOlyA(WT)

Plasmid

#132391

Purpose

Ostreolysin A for sensing sphingomyelin/cholesterol complexes

Depositor

Arun Radhakrishnan

Article

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A wild-type

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, C94S, S151C

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pDR119

Plasmid

#13332

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

DNA ligase IV (LIG4 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
SpyTag003-mKate2

Plasmid

#133452

Purpose

Expresses SpyTag003-mKate2 (a far-red fluorescent protein) in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyTag003-mKate2

Use

Tags

GSGSGS linker and His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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HECTD1

Plasmid

#32860

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HECTD1 (HECTD1 Human)

Use

Tags

His (with TEV cleavage)

Expression

Bacterial

Mutation

amino acids 1879-1966 only

Promoter

Available since

Jan. 6, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pCDB302

Plasmid

#113673

Purpose

expresses Cth SUMO protease in Escherichia coli

Depositor

Christopher Bahl

Article

Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

Insert

Cth SUMO protease

Use

Tags

10-His

Expression

Bacterial

Mutation

deleted amino acids 1-934

Promoter

T7

Available since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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NW11

Plasmid

#133443

Purpose

Covalently circularized nanodisc NW11 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pET-21a(+)-IPP1-His

Plasmid

#118978

Purpose

Bacterial expression plasmid for Inorganic pyrophosphatase

Depositor

Sebastian Maerkl

Article

Lavickova et al ACS Synth Biol. 2019 Jan 11. doi: 10.102

Insert

inorganic diphosphatase IPP1 (IPP1 Budding Yeast)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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LM627

Plasmid

#191551

Purpose

LM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-his

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Use

Tags

SNAC-tag, HIS-tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET16_Caspase-4_p10

Plasmid

#214308

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (CASP4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Use

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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LM670

Plasmid

#191552

Purpose

LM670_MSG-AGGA-promoter-ccdb-TTCC-GShis

Depositor

David Baker

Article

Wicky et al Science. 2022 Oct 7;378(6615):56-61. doi

Insert

ccdb

Use

Tags

HIS-tag

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NW30

Plasmid

#133444

Purpose

Covalently circularized nanodisc NW30 for studying membrane proteins and viral entry.

Depositor

Gerhard Wagner

Article

Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

Insert

NW30

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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SurA

Plasmid

#213138

Purpose

Expresses chaperone SurA

Depositor

Sebastian Hiller

Article

Rath et al Nat Commun. 2023 Sep 13;14(1):5648. doi:

Insert

SurA (21-428) (surA E.coli)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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6CCR (GID4)

Plasmid

#119960

Purpose

Bacterial expression for structure determination

Depositor

Cheryl Arrowsmith

Insert

GID4 (C17orf39 Human)

Use

Tags

6xHIS, thrombin,SUMO and TEV cleavage

Expression

Bacterial

Mutation

contains amino acids 116-300

Promoter

T7

Available since

Jan. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAG247 His-iFAST

Plasmid

#130808

Purpose

Expresses His-tagged iFAST in bacteria

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRAF-MutL

Plasmid

#86607

Purpose

E. coli MutL expression plasmid

Depositor

Richard Fishel

Article

Liu et al Nature. 2016 Nov 16;539(7630):583-587. d

Insert

E. coli MutL

Use

Tags

his-tag and ald-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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