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pv6_dPC_2xChromo

Plasmid

#179398

Purpose

Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of dPC_2xChromo

Depositor

Tuncay Baubec

Article

Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

Insert

dPC_2xChromo

Use

Tags

AviTag and EGFP

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR mCherry-Bcd-LEXY

Plasmid

#182596

Purpose

Drosophila integration plasmid expressing mCherry-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Use

Tags

LEXY and mCherry

Expression

Insect

Mutation

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBABR Bcd-LEXY

Plasmid

#182594

Purpose

Drosophila integration plasmid expressing Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bicoid (bcd Fly)

Use

Tags

LEXY

Expression

Insect

Mutation

Promoter

Available since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_C_FH_VIR

Plasmid

#186660

Purpose

Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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GLE_sgRNA

Plasmid

#186658

Purpose

GLE sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Gle sgRNA Plasmid (CG14749 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Shu

Plasmid

#186655

Purpose

Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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piwi_sgRNA

Plasmid

#186653

Purpose

piwi sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Piwi sgRNA Plasmid (piwi Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Donor_N_FH_Piwi

Plasmid

#186651

Purpose

Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)

Use

Tags

3xFlag-3xHA

Expression

Insect

Mutation

Promoter

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmPaip2-V5His6_Q

Plasmid

#147284

Purpose

Insect Expression of DmPaip2

Depositor

Elisa Izaurralde

Insert

DmPaip2

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmPaip1_G

Plasmid

#146353

Purpose

Insect Expression of DmPaip1

Depositor

Elisa Izaurralde

Insert

DmPaip1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmDCP1_128-372 (MCSI)_E

Plasmid

#146174

Purpose

Bacterial Expression of DmDcp1_128-372

Depositor

Elisa Izaurralde

Insert

DmDcp1_128-372 (Dcp1 Fly)

Use

Tags

Expression

Bacterial

Mutation

3 amino acid del (PQQ 318-320)

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmXRN1_1323-1383-DmDCP1_1-127_N

Plasmid

#147056

Purpose

Bacterial Expression of DmXRN1_1323-1383-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1383-DmDCP1_1-127 (pcm Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmDCP1-Y34A_P

Plasmid

#147214

Purpose

Insect Expression of DmDCP1-Y34A

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-Y34A (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-N41AS100A-dsRNAres_P

Plasmid

#147215

Purpose

Insect Expression of DmDCP1-N41AS100A-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-N41AS100A-dsRNAres (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-Y34F-dsRNAres_P

Plasmid

#147217

Purpose

Insect Expression of DmDCP1-Y34F-dsRNAres

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmDCP1-Y34F-dsRNAres (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuetG-DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127_P

Plasmid

#147220

Purpose

Bacterial Expression of DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127

Depositor

Elisa Izaurralde

Article

Braun et al Nat Struct Mol Biol. 2012 Dec;19(12):132

Insert

DmXRN1_1323-1355-L1323AQ1326A-DmDCP1_1-127 (pcm Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-D114AR115A-dsRNAres_Q

Plasmid

#147265

Purpose

Insect Expression of DmDCP1-D114AR115A-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmDCP1-D114AR115A-dsRNAres (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-F36A-dsRNAres_Q

Plasmid

#147266

Purpose

Insect Expression of DmDCP1-F36A-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmDCP1-F36A-dsRNAres (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-K125D-dsRNAres_Q

Plasmid

#147267

Purpose

Insect Expression of DmDCP1-K125D-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmDCP1-K125D-dsRNAres (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-HA-DmDCP1-R57D-dsRNAres_Q

Plasmid

#147268

Purpose

Insect Expression of DmDCP1-R57D-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmDCP1-R57D-dsRNAres (Dcp1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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