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Plasmid#123967PurposeEncodes the hROSA26 BxBI recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInserthRosa26-BxBI landing pad
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsub(TA)DD-Venus-NES
Plasmid#84631PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsub(TA)DD-Venus-NES
UseTags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationMutated target Thr residue to Ala.PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
JPF0454
Plasmid#124039PurposeEncodes pPGK1 driving expression of spCas9 P2A mAzamiGreen in a 2nd gen. Lenti Virus destination vectorDepositorInsertpHR_pPGK1-spCAS9-P2A-mAzamiGreen
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK8b_009
Plasmid#123896PurposeEncodes the 3' 500 bp hThal5.10-2-hg18 locus homology arm as a type 8b part to be used in the MTK systemDepositorInsertLA_hThal5.10-2-hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_027
Plasmid#123907PurposeEncodes the spCas9 sgRNA1 hThal5.10-2_hg18 (site 1) gRNA expression cassette as a type 234 part to be used in the MTK systemDepositorInsertsgRNA1 -hThal5.10-2_hg18
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-NNQ-HA-GFP
Plasmid#121164PurposeExpresses mmPtch1-B NNQ variantDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, N…PromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP
Plasmid#120909PurposeExpresses Ptch1-B (I766FV111FL114F)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_001
Plasmid#123924PurposeEncodes the spCas9 gRNA GFP dropout expression cassette with ConLS and ConRE connectors with Ampicillin resistance as a type 0 part to be used in the MTK systemDepositorInsertTU-sgRNA - L1/RE
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_049
Plasmid#123946PurposeEncodes the hThal5.10-2_hg18 landing pad destination vector as a type 0 part to be used in the MTK systemDepositorInserthThal5.10-2_hg18 landing pad destination vector
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only