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Plasmid#207762PurposeDonor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-HP1α-miRFP670-Cry2WT
Plasmid#122442PurposeExpresses disordered protein HP1α fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertHP1 alpha (CBX5 Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-NFIB-T2A-miRFP670
Plasmid#187222PurposeExpresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertNuclear Factor I B (NFIB Human)
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR26-CAG-H2B-miRFP703
Plasmid#112853PurposeDonor vector for targeting H2B-miRFP703 into Rosa26 locus to generate reporter mice or cell linesDepositorInsertH2B-miRFP703
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP713-HO1
Plasmid#197255PurposeExpresses the protein of miRFP713 and HO1 in bacteriaDepositorInsertmiRFP713
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P1-LoxP-Neo-N-miRFP670-C
Plasmid#159434PurposeCRISPR knock-in donor construct with fluorescent reporter and removable selectable markerDepositorInsertsNeomycin
miRFP670
UseCRISPRTags3' linker pair 1, 5' linker pair 1, C t…ExpressionMutationPromoterSV40Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-hCas9-T2A-miRFP670
Plasmid#187223PurposeExpresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.DepositorInsertCas9
UseTagsInserted T2A-miRFP670 at 3' terminal of MCS.ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miRFP680-HO1
Plasmid#197253PurposeExpresses the protein of miRFP680 and HO1 in bacteriaDepositorInsertmiRFP680
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF11-wmiRFP2-T2A-HO1
Plasmid#179485PurposeExpresses miRFP2 and HO1in worm cellsDepositorInsertwmIRFP2-T2A-HO1
UseTagsExpressionMammalianMutationNOPromotertag-168 promoterAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-miRFP670nano3-Blast-H3C2
Plasmid#207784PurposeDonor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780DepositorInsertH3C2 Homology Arms flanking a miRFP670nano3-Blast Cassette (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHR-TAF15N-miRFP670-Cry2WT
Plasmid#122440PurposeExpresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertTAF15 (TAF15 Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationContains only amino acids 1-208PromoterSFFVAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-FUSN-miRFP670-Cry2WT
Plasmid#122441PurposeExpresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertFUS (FUS Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationContains only amino acids 1-214PromoterSFFVAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE-miRFP703_PGK-Puro
Plasmid#216667PurposeExpresses tTa-inducible miRFP703, with puromycin resistanceDepositorInsertsmiRFP703
PuroR
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-miRFP670nano3-H3C2
Plasmid#227335PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780)DepositorInsertH3C2 Homology Arms flanking a miRFP670nano3 Tag (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-miRFP670nano3-H2BC11
Plasmid#227331PurposeDonor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)DepositorInsertH2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-9YS-miRFP670-TRF1
Plasmid#227381PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 9YS (TERF1 Human)
UseLentiviralTagsExpressionMutation9YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-27YS-miRFP670-TRF1
Plasmid#227383PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 27YS (TERF1 Human)
UseLentiviralTagsExpressionMutation27YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-15YS-miRFP670-TRF1
Plasmid#227382PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 15YS (TERF1 Human)
UseLentiviralTagsExpressionMutation15YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-5YS-miRFP670-TRF1
Plasmid#227380PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 5YS (TERF1 Human)
UseLentiviralTagsExpressionMutation5YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUS-3YS-miRFP670-TRF1
Plasmid#227379PurposeAdhesion module, creates 'seed' at telomereDepositorInsertTelomeric FUS IDR, 3YS (TERF1 Human)
UseLentiviralTagsExpressionMutation3YSPromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only