-
Plasmid#100864PurposeMammalian expression of mouse SPDEF truncationDepositorInsertSpdef (Spdef Mouse)
UseTagsEGFPExpressionMammalianMutationtruncated protein 85-325aaPromoterCMVAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mSPDEF deltaN3
Plasmid#100863PurposeMammalian expression of mouse SPDEF truncationDepositorInsertSpdef (Spdef Mouse)
UseTagsEGFPExpressionMammalianMutationtruncated protein 70-325aaPromoterCMVAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-mSPDEF deltaN2
Plasmid#100862PurposeMammalian expression of mouse SPDEF truncationDepositorInsertSpdef (Spdef Mouse)
UseTagsEGFPExpressionMammalianMutationtruncated protein 55-325aaPromoterCMVAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3_zfh1_CP-candidate_luc+
Plasmid#86391PurposeLuciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates responsiveness to the upstream enhancer is reflected by luciferase activityDepositorInsertD. melanogaster zfh1 enhancer
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
RP185 pUAST EGFP:Or47b
Plasmid#59525PurposeN-terminal fusion of GFP to Drosophila melanogaster Or47bDepositorInsertEGFP:Or47b (Or47b Fly)
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
UseTagsExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS-C
Plasmid#58326PurposeDestination vector for inserting ubi-CadTS control to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor control
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
RP125 pUAST EGFP:Orco
Plasmid#53441PurposeN-terminal fusion of EGFP to Drosophila melanogaster OrcoDepositorInsertEGFP:Orco (Orco Fly)
UseTagsEGFPExpressionInsectMutationPromoterAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-GFP-RpL10Ab
Plasmid#71729PurposeExpression of Drosophila melanogaster Rpl10Ab-GFP fusionDepositorInsertRpL10Ab (RpL10Ab Fly)
UseTagsEGFPExpressionInsectMutationPromoterhsp70Available sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2-IRES-dOrai
Plasmid#67627PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOraiDepositorInsertdmBACCS2-IRES-dOrai
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
UAS::PTPRFb-DN-GFP
Plasmid#37111DepositorInsertUAS::PTPRFb-DN-GFP (ptprfb Zebrafish)
UseTol2 gateway expression vectorTagsEGFPExpressionMutationDeleted aa1386-1909 corresponding to phosphatase …Promoter10xUASAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
mRFP-dmBACCS2
Plasmid#67630PurposeDrosophila expression of mRFP-dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switchDepositorInsertmRFP-dmBACCS2
UseDrosophila expressionTagsExpressionMutationPromoterDrosophila actin gene 5C gene promoterAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-Venus
Plasmid#58328PurposeDestination vector for inserting ubi-Cad-Venus to Drosophila melanogaster genomeDepositorInsertCad-Venus
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUWR-CadTS
Plasmid#58325PurposeDestination vector for inserting ubi-CadTS to Drosophila melanogaster genomeDepositorInsertE-cadherin tension sensor
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-zfh1
Plasmid#86381PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster zfh1 enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUWR-Cad-mTFP
Plasmid#58327PurposeDestination vector for inserting ubi-Cad-mTFP to Drosophila melanogaster genomeDepositorInsertCad-mTFP
UseTagsExpressionInsectMutationPromoterpoly-ubiquitinAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER20-Flag-NLS-hSPDEF delta1
Plasmid#100874PurposeLentiviral expression of human SPDEF truncationDepositorInsertSPDEF (SPDEF Human)
UseLentiviralTags3xFLAGExpressionMammalianMutationtruncated protein 1-248aaPromoterTRE2Available sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_fly-tj
Plasmid#86386PurposeVector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.DepositorInsertD. melanogaster tj enhancer
UseStap-seq screening vectorTagsExpressionMutationPromoterAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1_GST-DmKHC[1-421]-1xGFP
Plasmid#196973PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and cleavable N-terminal GST tagDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGFP and GSTExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-1_GST-SNAP-DmKHC[1-421]
Plasmid#196976PurposeBacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with N-terminal SNAP tag and cleavable N-terminal GSTDepositorInsertKinesin Heavy Chain (Khc Fly)
UseTagsGST and SNAPExpressionBacterialMutationPromotertacAvailable sinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only