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Plasmid#81198PurposepTIM in vitro transcription backbone plasmid with two multiple cloning sites and two Rho-independent transcriptional terminators.DepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only
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pVDL9.3
Plasmid#168299PurposeSecretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyADepositorInsertSegment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
UseTagsExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-SopE
Plasmid#221327PurposeDox-inducible expression of SopE in mammalian cells by retroviral transductionDepositorInsertSopE
UseRetroviralTagsFLAGExpressionMutationPromoterTRE3GVAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2SCb
Plasmid#59385PurposeTemplate plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.DepositorInsertTT-ISceI-cat2
UseTemplateTagsExpressionMutationThe 3’-end of the chloramphenicol resistance gene…PromoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pWJB9
Plasmid#126650PurposeExpresses AdrA from a modified pLAFR3DepositorInsertadrA-8xHis/AdrA-8xHis including flanking promoter regions from pBAD30
UseTags8xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer-stm2585
Plasmid#174395PurposeConstitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)DepositorInsertIL6ST cytosolic domain and sarA chimera
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585
Plasmid#174391PurposeS. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tagDepositorInsertstm2585
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM8731
Plasmid#213991PurposePrpsM driven expression of mNeonGreen - sequence codon-optimized for SalmonellaDepositorInsertmNeonGreen
UseTagsExpressionBacterialMutationPromoterPrpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-gp130dimer
Plasmid#174394PurposeCytosolic domain of gp130 with C-C linker and Leu zipper for constitutive dimerization and activation (from Stuhlmann-Laeisz et al. 2006).DepositorInsertIL6ST cytosolic domain with leucine zipper & C-C linker
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSens-4OmeNB
Plasmid#216231PurposeExpresses GFP in response to 4'-O-MethylnorbelladineDepositorInsertsRamR-4NB2
sfGFP
UseSynthetic BiologyTagsExpressionMutationK64T, L67M, C135D, S138GPromoterJ23103 and PramrAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDL14
Plasmid#106901PurposepDL14 is conditionally replicating plasmid and expresses gam, bet and mutSK622A genes under control of PrhaB promoter for oligo-mediated E. coli chromosome engineering in MMR independent mannerDepositorInsertsgam
bet
mutS
lacI
rhaR
rhaS
UseTagsExpressionBacterialMutationLysine 622 was replaced by AlaninePromoterAvailable sinceJune 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-stm2585-gp130
Plasmid#174393PurposeChimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.DepositorInsertsarA-IL6ST chimera
UseTagsFlagExpressionMammalianMutation140-177 of sarA replaced with homologous IL6ST se…PromoterAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-AcGFP-Sec61-IRES-Blast
Plasmid#154009PurposeLentiviral vector plasmid for integration and constitutive expression of AcGFP fused to Sec61 in mammalian cells – gift from D. Gerlich (IMBA, Vienna)DepositorInsertacGFP-Sec61 (SEC61B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-InC
Plasmid#83959PurposeContains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.DepositorInsertInternal Control sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mCherry-G12V-KRAS-IRES-Blast
Plasmid#153336PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to oncogenic G12V-KRAS in mammalian cellsDepositorInsertmCherry-G12V-KRAS (KRAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-mCherry-WT-KRAS-IRES-Blast
Plasmid#153335PurposeLentiviral vector plasmid for integration and constitutive expression of mCherry fused to wild-type KRAS in mammalian cellsDepositorInsertmCherry-WT-KRAS (KRAS Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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