Addgene

Showing: 201 - 220 of 454 results

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pAAV-MCU-EGFP

Plasmid

#181866

Purpose

Expresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoter

Depositor

Hilmar Bading

Article

Qiu et al Nat Commun. 2013;4:2034. doi: 10.1038/nc

Insert

mitochondrial calcium uniporter (Mcu Mouse)

Use

AAV and Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CaMKIIa

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

Plasmid

#174893

Purpose

amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Insert

mCherry and EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense

Plasmid

#46835

Purpose

Express cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense

Plasmid

#46836

Purpose

Express cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

Plasmid

#46837

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

Plasmid

#46838

Purpose

Express cGFP transcript ending in the minimal functional MALAT1 triple helix

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

Plasmid

#46839

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Ma-sfGFP WT

Plasmid

#197567

Purpose

Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
M. alvus Pyl-tRNA (6) (4x copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

Promoter

CMV and U6/H1

Availability

Academic Institutions and Nonprofits only

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pAcBac1-Mb-sfGFP WT

Plasmid

#197569

Purpose

Expression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
Pyl-tRNA (4 copies)

Use

Tags

V5-His6

Expression

Mammalian

Mutation

Promoter

CMV and U6/H1

Availability

Academic Institutions and Nonprofits only

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pALS2-sfGFP WT

Plasmid

#197575

Purpose

Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replication

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP WT
M. alvus Pyl-tRNA (6)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

araC and lpp

Availability

Academic Institutions and Nonprofits only

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ARR3tk-eGFP/SV40-mCherry

Plasmid

#132360

Purpose

To measure transcriptional activity of Androgen Receptor

Depositor

Charles Sawyers

Article

Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

Insert

AR responsive elements (AR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSAD-5PSD95-EGFP-SynPhRFP

Plasmid

#217979

Purpose

G-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFP

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Inserts

5PSD95-EGFP (Dlg4 Rat)
SynPhRFP (Syp Mouse)

Use

G-deleted rabies

Tags

EGFP and RFP

Expression

Mutation

Promoter

None

Availability

Academic Institutions and Nonprofits only

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MCS-T2a-GFPnls-ER-Cre-ER

Plasmid

#200104

Purpose

Vector For Twist

Depositor

Prayag Murawala

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pLV-TetO-hNGN2-eGFP-Puro

Plasmid

#79823

Purpose

Expresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.

Depositor

Kristen Brennand

Article

Ho et al Methods. 2015 Nov 25. pii: S1046-2023(15

Insert

hNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TetO

Availability

Academic Institutions and Nonprofits only

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pALS2-sfGFP 150TAG

Plasmid

#197574

Purpose

Fluorescence selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-150TAG and M. alvus Pyl-tRNA(6). p15a origin of replication.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Inserts

sfGFP 150TAG
M. alvus Pyl-tRNA (6)

Use

Tags

His6

Expression

Bacterial

Mutation

N150TAG

Promoter

araC and lpp

Availability

Academic Institutions and Nonprofits only

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MSCV-dGFP-JARID1C A388P

Plasmid

#74778

Purpose

retroviral expression of JARID1C A388P

Depositor

Giovanni Tonon

Article

Rondinelli et al J Clin Invest. 2015 Dec;125(12):4625-37.

Insert

JARID1C (KDM5C Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

A388P

Promoter

Availability

Academic Institutions and Nonprofits only
pAAV-APLP1ICD-IRES-hrGFP

Plasmid

#107546

Purpose

AAV-mediated expression of last 50 amino acids of APLP1 protein (APLP1ICD) and IRES-mediated co-expression of hrGFP to recognize labelled cells

Depositor

Hélène Marie

Article

Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

Insert

APLP1ICD (APLP1 Human)

Use

AAV

Tags

Expression

Mutation

None

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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sgGFP_3

Plasmid

#78165

Purpose

sgGFP

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

GFP

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Availability

Academic Institutions and Nonprofits only
pRC54-DHX37-GFP

Plasmid

#192149

Purpose

express DHX37-GFP

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Insert

DHX37-GFP (Dhx37 Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Availability

Academic Institutions and Nonprofits only

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pRC55-GFP-DHX37

Plasmid

#192150

Purpose

express GFP-DHX37

Depositor

Sidi Chen

Article

Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

Insert

GFP-DHX37 (Dhx37 Mouse)

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Availability

Academic Institutions and Nonprofits only

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