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Plasmid#121164PurposeExpresses mmPtch1-B NNQ variantDepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291, N…PromoterCMVAvailable sinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP
Plasmid#120909PurposeExpresses Ptch1-B (I766FV111FL114F)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFP
Plasmid#120908PurposeExpresses Ptch1-B (V111FL114FW115A)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-I766FV111F-HA-GFP
Plasmid#120907PurposeExpresses Ptch1-B (I766FV111F)DepositorInsertPtch1 (Ptch1 Mouse)
UseTagsGFP and HAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-MCU-EGFP
Plasmid#181866PurposeExpresses GFP-tagged mitochondrial calcium uniporter (MCU) under control of the CaMKIIa promoterDepositorInsertmitochondrial calcium uniporter (Mcu Mouse)
UseAAV and AdenoviralTagsEGFPExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
SAM-DNMT3A+sgGFP
Plasmid#213165PurposeVector with sgGFP for induction of global DNA methylation.DepositorInsertsgGFP
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterE1Fa and U6 and PGKAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP
Plasmid#174893Purposeamber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassetteDepositorInsertmCherry and EGFP
UseTagsExpressionMammalianMutationPromoterEF1Available sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense
Plasmid#46835PurposeExpress cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]DepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-SV40 Poly(A) Sense
Plasmid#46836PurposeExpress cGFP transcript ending in a poly(A) tail [using the SV40 poly(A) signal]DepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense
Plasmid#46839PurposeExpress cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translatedDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense
Plasmid#46838PurposeExpress cGFP transcript ending in the minimal functional MALAT1 triple helixDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense
Plasmid#46837PurposeExpress cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degradedDepositorInsertCoral Green Fluorescent Protein (cGFP)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pALS2-sfGFP WT
Plasmid#197575PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin of replicationDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6)
UseTagsHis6ExpressionBacterialMutationPromoteraraC and lppAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Mb-sfGFP WT
Plasmid#197569PurposeExpression of sfGFP WT with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
Pyl-tRNA (4 copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-sfGFP WT
Plasmid#197567PurposeExpression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cellsDepositorInsertssfGFP WT
M. alvus Pyl-tRNA (6) (4x copies)
UseTagsV5-His6ExpressionMammalianMutationPromoterCMV and U6/H1Available sinceApril 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
ARR3tk-eGFP/SV40-mCherry
Plasmid#132360PurposeTo measure transcriptional activity of Androgen ReceptorDepositorInsertAR responsive elements (AR Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SAM-DNMT3A-inactive+sgGFP
Plasmid#213169PurposeVector with sgGFP used as a negative control (inactive DNMT3A) for induction of global DNA methylation.DepositorInsertsgGFP
UseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterE1Fa and U6Available sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAD-5PSD95-EGFP-SynPhRFP
Plasmid#217979PurposeG-Deleted Rabies genomic plasmid to express 5PSD95-EGFP and SynPhRFPDepositorUseG-deleted rabiesTagsEGFP and RFPExpressionMutationPromoterNoneAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCS-T2a-GFPnls-ER-Cre-ER
Plasmid#200104PurposeVector For TwistDepositorTypeEmpty backboneUseUnspecifiedTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-TetO-hNGN2-eGFP-Puro
Plasmid#79823PurposeExpresses human NEUROGENIN2 (hNGN2), eGFP and puromycin resistance gene under control of TetON promoter. This 3rd generation lentiviral vector is used to generate NGN2-iNs from hiPSCs and hiPSC-NPCs.DepositorInserthNGN2-P2A-eGFP-T2A-PuroR (NEUROG2 Human)
UseLentiviralTagsExpressionMutationPromoterTetOAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only