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pMGF173

Plasmid

#96999

Purpose

NLS-GFP-lacZ leu1 insertion vector

Depositor

Adam Frost, Wesley Sundquist

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

lacZ

Use

Tags

NLS-GFP

Expression

Yeast

Mutation

Promoter

Pnmt1

Available since

Aug. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pCSFLPe

Plasmid

#31130

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

FLP recombinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MAT2A

Plasmid

#100521

Purpose

Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

MAT2A (MAT2A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

pTight TRE

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLC242-GFP-metap2

Plasmid

#100518

Purpose

Expresses GFP tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-metap2

Plasmid

#100510

Purpose

Expresses FLAG tagged metap2 in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

METAP2 (METAP2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-metap2

Plasmid

#100512

Purpose

Expresses HA tagged metap2 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLJC5-Flag-SAMTOR

Plasmid

#102420

Purpose

3rd generation lentiviral vector expressing FLAG SAMTOR in mammalian cells driven by UBC promoter

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-metap2

Plasmid

#100516

Purpose

Expresse Myc tagged metap2 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

Metap2 (METAP2 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SAMTOR (G172A)

Plasmid

#100514

Purpose

Expresses HA tagged SAMTOR G172A mutant in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

G172A

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SAMTOR (D190A)

Plasmid

#100515

Purpose

Expresses HA tagged SAMTOR D190A mutant in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

D190A

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLC242-GFP-SAMTOR

Plasmid

#100520

Purpose

Expresses GFP tagged SAMTOR in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/FLAG-hPMR1-Tev-Bio

Plasmid

#80125

Purpose

Expresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

Depositor

Daniel Schoenberg

Article

Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

Insert

PXDNL-003 (PXDNL Human)

Use

Tags

FLAG and biotinylation

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-FLAG-Kaptin

Plasmid

#100511

Purpose

Expresses FLAG tagged Kaptin in mammalian cells to make lentivirus

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

KPTN (KPTN Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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HS_NAM-B1

Plasmid

#154064

Purpose

Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Inserts

Hygromycin phosphotransferase
Cre recombinase
LoxP-flanked GUS and TtNAM-B1

Use

Cre/Lox and Synthetic Biology; Golden gate

Tags

Expression

Bacterial and Plant

Mutation

The TtNAM-B1 gene sequence was domesticated to re…

Promoter

HvHSP17, OsAct, and ZmUbi

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV:DsRed(FRT)GFP

Plasmid

#31128

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

DsRed(FRT)GFP

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV:GFP(FRT)DsRed

Plasmid

#31372

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(FRT)DsRed

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV:GFP(loxP)lacZ

Plasmid

#31125

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(loxP)lacZ

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV:GFP(FRT)lacZ

Plasmid

#31124

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(FRT)lacZ

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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LCMV:ECFP(loxP)MCS

Plasmid

#31376

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

ECFP(loxP)MCS

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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LCMV:GFP(FRT)MCS

Plasmid

#31298

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(FRT)MCS

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only

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