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Plasmid#222937PurposeKill switch plasmid for DNA sensor construction using Bacillus subtilisDepositorInsertTxpA-RatA toxin-antitoxin
UseTagsExpressionBacterialMutationPromoterPhyperspankAvailable sinceDec. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBz118_His_CsoS2
Plasmid#140841PurposeHalothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backboneDepositorInsertCsoS2
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
Smp_031880-Cd4-bio-His
Plasmid#120602PurposeExpression of recombinant S. mansoni proteinDepositorArticleInsertSmp_031880 (Smp_031880 )
UseTagsratCd4(d3+4)-bio-6HisExpressionMammalianMutationPromoterCMVAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLp_3050sNuc
Plasmid#122030PurposePlasmid for heterologous protein secretion in Lactobacillus plantarum and L. sakeiDepositorInsertsignal peptide Lp_3050
UseTagsExpressionBacterialMutationPromotersppAAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBintE
Plasmid#48136PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDSW1728
Plasmid#120812PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)DepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPO2
Plasmid#209976PurposeContains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmidsDepositorInsertreplication origin (SH71_rep)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPO4
Plasmid#209978PurposeContains Level 0 Part: replication origin (pWV01) for the construction of Level 1 plasmidsDepositorInsertreplication origin (pWV01)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPR3
Plasmid#209986PurposeContains Level 0* Part: resistance cassette (ermBL) for the construction of Level 2 plasmidsDepositorInsertresistance cassette (ermBL)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPS2
Plasmid#209972PurposeContains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmidsDepositorInsertRBS (Lp)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLPR1
Plasmid#209979PurposeContains Level 0 Part: resistance cassette (ermBL) for the construction of Level 1 plasmidsDepositorInsertresistance cassette (ermBL)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJAK112
Plasmid#167280PurposeCodA-based mutagenesis vector for C. difficileDepositorTypeEmpty backboneUseE. coli - c. difficile shuttle vectorTagsExpressionMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAD-sfGFP(Sp)-Pman
Plasmid#133833PurposeFluorescent protein sfGFP(Sp); under control of mannose inducible promoter.DepositorInsertsfGFP(Sp)
UseE.coli/bacillus spp. shuttle vectorTagsExpressionBacterialMutationPromoterPmanAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBintT
Plasmid#48134PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker tetracycline for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHBintN
Plasmid#48135PurposeShuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker neomycine for B. megaterium, integration vectorDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pBz15_Strep_Rubisco
Plasmid#140839Purposewild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backboneDepositorInsertForm IA Rubisco; CbbL, CbbS
UseTagsStrep IIExpressionBacterialMutationPromoterT7Available sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJK413
Plasmid#155372PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus] (sgRNAs flip of 412)DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only