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Plasmid#147455PurposeMammalian Expression of HsEDC4. Please note that this plasmid does not contain the T7 promoter.DepositorInsertHsEDC4 (EDC4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pg-ODD-EGFP
Plasmid#87211PurposeHIF-1a's ODD domain fused to EGFPDepositorInsertHIF-1a ODD domain (HIF1A Human)
UseTagsEGFPExpressionMammalianMutationODD domain (AA 530-652)PromoterCMVAvailable sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK172-α-syn-TEV-GFP
Plasmid#166671PurposeBacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studiesDepositorInsertmouse synuclein (from V. Lee) (Snca Mouse)
UseTagsGFPExpressionBacterialMutationadded a TEV cleavage site sequence between synucl…PromoterCMVAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2
Plasmid#112837Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationSH2 + SH3 (C-term) domains of Grb2PromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-5
Plasmid#112840Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationFull length Grb2PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-4
Plasmid#112839Purposetransient/stable overexpressionDepositorInsertGrb2 (Grb2 Mouse)
UseTagsGFPExpressionMammalianMutationSH3 (N-term) domain of Grb2PromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-hSNCA-GFP
Plasmid#215489PurposeLentiviral expression and easy visualization of alpha-synuclein in mammalian cellsDepositorInsertalpha-synuclein (SNCA Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterUbcAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcry:GFP,-503unc:hACTA1GFP
Plasmid#172529PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The 503bp unc-45b muscle-specific promoter drives expression of GFP-tagged human ACTA1.DepositorInsertATCA1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2/shαSMA/GFP4_Seq2/clone1
Plasmid#201402PurposeKnockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertalpha-SMA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHIV-ODD-EGFP-IRES-dTomato
Plasmid#87218Purposelentiviral expression of ODD-EGFPDepositorInsertHIF-1a ODD domain (HIF1A Human)
UseLentiviralTagsEGFPExpressionMammalianMutationODD domainPromoterEF1Available sinceMarch 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1.1-eGFP
Plasmid#162738PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish aBb crystallin 5kb promoter/AcGFP
Plasmid#98101PurposeZebrafish aBb crystallin 5kb promoter segment driving GFP expressionDepositorInsertAlpha Bb-crystallin promoter 5 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Bb-crystallin 5 kb promoter fragment …Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish aBb crystallin 4kb promoter/AcGFP
Plasmid#98100PurposeZebrafish aBb crystallin 4kb promoter segment driving GFP expressionDepositorInsertAlpha Bb-crystallin promoter 4 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Bb-crystallin 4 kb promoter fragment …Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Zebrafish aBb crystallin 2kb promoter/AcGFP
Plasmid#98099PurposeZebrafish aBb crystallin 2kb promoter segment driving GFP expressionDepositorInsertAlpha Bb-crystallin promoter 2 kb fragment, Danio rerio (cryabb Zebrafish)
UseGfp expressingTagsGFPExpressionMutationPromoterDanio alpha Bb-crystallin 2 kb fragment (-2092/-1)Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 1.5kb promoter/AcGFP
Plasmid#98104PurposeMouse aB crystallin 1.5kb promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 1.5 kb fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPExpressionMutationPromoterAlpha B-crystallin 1.5 kb promoter fragment (-150…Available sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 250bp promoter/AcGFP
Plasmid#98102PurposeMouse aB crystallin 250bp promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 250 bp fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPExpressionMutationPromoterAlpha B-crystallin promoter 250 bp fragment (-259…Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
Mouse aB crystallin 800bp promoter/AcGFP
Plasmid#98103PurposeMouse aB crystallin 800bp promoter segment driving GFP expressionDepositorInsertAlpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)
UseGfp expressingTagsGFPExpressionMutationPromoterAlpha B-crystallin promoter 800 bp fragment (-833…Available sinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW-hSNCA(D2R)-GFP
Plasmid#215490PurposeLentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to arginine) in mammalian cellsDepositorInsertalpha-synuclein (SNCA Human)
UseLentiviralTagsEGFPExpressionMammalianMutationD (second aa) mutated to RPromoterUbcAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
FUW-hSNCA(D2E)-GFP
Plasmid#215491PurposeLentiviral expression and easy visualization of mutated alpha-synuclein (2nd amino acid aspartate mutated to glutamate) in mammalian cellsDepositorInsertalpha-synuclein (SNCA Human)
UseLentiviralTagsEGFPExpressionMammalianMutationD (second aa) mutated to EPromoterUbcAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Zeo-eGFP
Plasmid#162743PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only