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Plasmid#133307Purpose"Burdensome" GFP expressing plasmid carrying microcin-V bacteriocin for plasmid stabilisationDepositorInsertmicrocin-V bacteriocin cassette (cvaC E. coli)
UseSynthetic BiologyTagsExpressionBacterialMutationN112D (please see depositors comments)PromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC118-FLIP-Puro
Plasmid#84538Purposea mock vector for generating FLIP targeting vectorsDepositorInsertFLIP - Puro
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B5
Plasmid#172846PurposeCRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6K-mini-Tn7T
Plasmid#64958Purposemini-Tn7 base vector with transcriptional terminatorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19 Giles integrase
Plasmid#170049PurposepUC19 with Giles integraseDepositorInsertGIles Integrase
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7
Plasmid#64953Purposemini-Tn7 base vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18T-mini-Tn7T-Tp
Plasmid#65024PurposeTpR mini-Tn7 vector, mobilizableDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC57-white[coffee]
Plasmid#84006PurposeDonor HR template carrying a 2,080 bp fragment of the Drosophila melanogaster white[coffee] allele and silent mutations conferring resistance to white sgRNAs-1, -2, -3, and -4DepositorInsertA 2,080 bp fragment of the white[coffee] allele (w Fly)
UseUnspecifiedTagsExpressionMutationA GC-to-AA mutation that creates a G589E missense…PromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T-Gm-GW
Plasmid#64961Purposemini-Tn7 Gateway destination vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-YAP-mEGFP
Plasmid#166472PurposeHDR template for tagging of endogenous mouse YAP N-terminus with mEGFPDepositorInsertYAP-mEGFP HDR template(mouse Yap) (Yap1 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B1
Plasmid#172842PurposeCRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mEGFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-P70a-3WJdB-T
Plasmid#87311PurposeExpresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the E. coli sigma 70 promoter.DepositorInsertThree-way Junction dimeric Broccoli aptamer
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC57 iLOV CHLAMY
Plasmid#26769DepositorInsertiLOV
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B2
Plasmid#172843PurposeCRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mRuby3
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-HBB-nLuc
Plasmid#175432PurposeUsed to generate IVT RNA for translation extractsDepositorInsertHBB-nLuc
UseLuciferaseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18-mini-Tn7T
Plasmid#64954Purposemini-Tn7 base vector with transcriptional terminatorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUCBB-pT7-eGFP
Plasmid#32552DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionBacterialMutationPromoterpT7Available sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B4
Plasmid#172845PurposeCRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mNeonGreen
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B3
Plasmid#172844PurposeCRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mTurquoise2
UseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC57-GFP-SFPQ-RT
Plasmid#97090PurposeEncodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.DepositorInsertEGFP-SFPQ-RT (SFPQ Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B3
Plasmid#103004Purposenon-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B3 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-iCAP-PHP.B2
Plasmid#103003Purposenon-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification systemDepositorInsertSynthetic construct isolate AAV-PHP.B2 VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceDec. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC-DEST-R3R4(R)
Plasmid#141010PurposeMutisite Gateway mediated vector constructionDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18PV-mini-Tn7T
Plasmid#64956PurposepUC18-mini-Tn7T with unique PvuII site in pUC18 DNADepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19/mChe-Golgi
Plasmid#183169PurposeConstruct for transient expression of Golgi apparatus-targeted mCherry in plants.DepositorInsertmCherry-Golgi
UseTagsAtRER1B, A. thaliana endoplasmic reticulum retrie…ExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ntH6-eGFP
Plasmid#32557DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin siteExpressionBacterialMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-Aa-artsg13 (BsaI)
Plasmid#188279PurposeMammalian expression, Genome editingDepositorInsertAa-artsg13
UseCRISPRTagsExpressionMammalianMutationnonePromoterU6Available sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B28-Leum
Plasmid#44327DepositorInsertUAS1B28-Leum promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B28-LeumAvailable sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-UAS1B16-Leum
Plasmid#44324DepositorInsertUAS1B16-Leum promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B16-LeumAvailable sinceMay 23, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19 PKC delta
Plasmid#8427DepositorInsertMouse Protein Kinase C delta (Prkcd Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ptet-eGFP
Plasmid#32554DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterptetAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-pBAD-eGFP
Plasmid#32553DepositorInserteGFP (eGFP )
UseSynthetic BiologyTagsHisExpressionMutationPromoterpBADAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUC19 PKC zeta
Plasmid#8425DepositorInsertMouse Protein Kinase C zeta (Prkcz Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mlcR25-mEGFP
Plasmid#154295PurposeFluorescent reporter for conony screening without an inducer (e.g. IPTG); TED module (Plac-lacO-mlcR25-mEGFP)DepositorInsert25-bp fragment of the 3′ end of mlcR (mlcR25); mEGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19 PKC alpha
Plasmid#8429DepositorInsertMouse Protein Kinase C alpha (Prkca Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
SRAcode_in_pUC57_v3(767-1356)
Plasmid#49826Purposefor transcribing SRA nucl 767-1356 of NM_001035235 with T7DepositorInsertsteroid receptor RNA activator RNA coding (SRA1 Human)
UseTagsExpressionBacterialMutationKpnI site at 5' start of RNA coding seqPromoterT7Available sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-Leum
Plasmid#44316DepositorInsertUAS1B8-Leum promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-LeumAvailable sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19/Mito-mChe
Plasmid#183167PurposeConstruct for transient expression of mitochondrion-targeted mCherry in plants.DepositorInsertMito-mCherry
UseTagsOsCOX11, Oryza sativa cytochrome c oxidase assemb…ExpressionPlantMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAW79.pUC19.noYY1
Plasmid#104377PurposeNo YY1 binding motifs as control for in vitro ligationDepositorInsertNo YY1 binding motifs
UseTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAW49.pUC19.YY1
Plasmid#104376PurposeContains YY1 binding sites for in vitro ligationDepositorInsertYY1 binding motifs
UseTagsExpressionMutationPromoterAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_AVR-Pia
Plasmid#123653PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertAVR-Pia
UseGolden gateTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_TH16_AVR-Pib_100
Plasmid#121378PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertTH16_AVR-Pib_100
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_TH12-rn_00121641
Plasmid#121371PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertTH12-rn_00121641
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Kan_PH14-rn_00186401
Plasmid#121369PurposeLevel 0 Golden-Gate compatible vectorDepositorInsertPH14-rn_00186401
UseGolden gateTagsExpressionMutationMagnaporthe oryzaePromoterAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B8-TEF
Plasmid#44374DepositorInsertUAS1B8-TEF promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B8-TEFAvailable sinceApril 17, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC-mCA1-emptyMCS
Plasmid#35095DepositorTypeEmpty backboneUseMouse TargetingTagsExpressionMutationPromotermouse carbonic anhydrase 1Available sinceApril 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC57 iLOV ANAB
Plasmid#26770DepositorInsertiLOV
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B20-Leum
Plasmid#44325DepositorInsertUAS1B20-Leum promoter
UseSynthetic BiologyTagsExpressionBacterialMutationN/APromoterUAS1B20-LeumAvailable sinceApril 18, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19 PKC epsilon
Plasmid#8442DepositorInsertMouse Protein Kinase C epsilon (Prkce Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pUCBB-ctH6-eGFP
Plasmid#32562DepositorInserteGFP (eGFP )
UseSynthetic BiologyTags6 His tag with Thrombin siteExpressionMutationPromoterConsitutive lacAvailable sinceOct. 18, 2011AvailabilityAcademic Institutions and Nonprofits only