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Showing: 501 - 550 of 651 results

  6. pPPC011

    Plasmid
    #171143
    Purpose
    Expression of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) on pRK2-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available since
    Oct. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171143-plasmid-map-sequence-id-345216

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  8. pPPC001

    Plasmid
    #171138
    Purpose
    For integration of Sp.pCas9-dCas9 and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Insert
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    Sp.pCas9, BBa_J23107
    Available since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    171138-plasmid-map-sequence-id-344761

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  12. pYI2206

    Plasmid
    #228348
    Purpose
    Constitutive rPhlTA mutant expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228348-plasmid-map-sequence-id-457096

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  13. pMT957

    Plasmid
    #228293
    Purpose
    Constitutive mutant PhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    E41G
    Promoter
    ScPGK1 promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228293-plasmid-map-sequence-id-457079

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  14. pMT1026

    Plasmid
    #228299
    Purpose
    Constitutive expression of PhlTA mutant with weak promoter and terminator
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    E41G
    Promoter
    KpYPT1 promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228299-plasmid-map-sequence-id-457081

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  15. pMT478

    Plasmid
    #228286
    Purpose
    Constitutive mutant rPhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    rphlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228286-plasmid-map-sequence-id-457102

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  19. pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

    Plasmid
    #191651
    Purpose
    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4
    Depositor
    Emily Balskus
    Article
    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:
    Insert
    CymR
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    191651-plasmid-map-sequence-id-375980

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  22. pT18mobsacB

    Plasmid
    #72648
    Purpose
    sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 18, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72648-plasmid-map-sequence-id-399780

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  23. pDONR2T18ms

    Plasmid
    #72645
    Purpose
    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 29, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72645-plasmid-map-sequence-id-133017

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  24. pDEST2T18ms

    Plasmid
    #72647
    Purpose
    Gateway-compatible sucrose counter-selection allelic exchange vector for Pseudomonas syringae
    Depositor
    Brian Kvitko
    Type
    Empty backbone
    Use
    Sucrose counter-selection allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 10, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    72647-plasmid-map-sequence-id-139809

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  25. P20_992-tetO-deGFP

    Plasmid
    #97096
    Purpose
    Expresses GFP in the presence of Pseudomonas fluorescence sigma factor ECF20_992. Repressed by TetR
    Depositor
    Richard Murray
    Insert
    deGFP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P20_992 with TetO operator site
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    97096-plasmid-map-sequence-id-195303

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  29. pPPC010.AAV

    Plasmid
    #171175
    Purpose
    Expression of Sp.pCas9-dCas9, BBa_J23107-MCP-SoxS(R93A/S101A), and hAAVS1 scRNA on pBBR1-KmR plasmid
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    hAAVS1 scRNA
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    BBa_J23119 and Sp.pCas9, BBa_J23107
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171175-plasmid-map-sequence-id-344748

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  30. pMT899

    Plasmid
    #228288
    Purpose
    Constitutive mutant rPhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    rphlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228288-plasmid-map-sequence-id-457110

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  39. pKTop::sptse5-CT (1169-1229)

    Plasmid
    #192948
    Purpose
    For membrane insertion study; expresses Tse5-CT (1169-1229)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1229)
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1229)
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192948-plasmid-map-sequence-id-380616

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  43. pS238D1::sptse5-CT_phoA-lacZalpha

    Plasmid
    #192961
    Purpose
    Test the biological function of the spTse5-CT (1169-1317)-PhoA (22-474)-LacZ (4-60) fusion protein in P. putida
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT_phoA-lacZ_
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1317) fused to phoA-lacZal…
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192961-plasmid-map-sequence-id-380611

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  45. pKTop::sptse5-CT (1169-1281)

    Plasmid
    #192950
    Purpose
    For membrane insertion study; Expresses sptse5-CT (1169-1281) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1281)
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1281)
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192950-plasmid-map-sequence-id-380607

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  46. pKTop::sptse5-CT (1169-1269)

    Plasmid
    #192949
    Purpose
    For membrane insertion study; Expresses Tse5-CT (1169-1269)/PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1269)
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for tse5 (1169-1269)
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192949-plasmid-map-sequence-id-380605

    Enlarge

  47. pKTop::sptse5-CT (1169-1300)

    Plasmid
    #192951
    Purpose
    For membrane insertion study; Expresses sptse5-CT (1169-1300) /PhoA (22-472)/LacZ (4-60)fusion with PelB signalpeptide fused at the N-terminus
    Depositor
    David Albesa-Jové
    Article
    Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:
    Insert
    sptse5-CT (1169-1300)
    Use
    Tags
    PelB signal peptide
    Expression
    Bacterial
    Mutation
    encodes for sptse5 (1169-1300)
    Promoter
    Available since
    Dec. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    192951-plasmid-map-sequence-id-380604

    Enlarge

  48. pPPC002

    Plasmid
    #171139
    Purpose
    For integration of J1-J23117-sfGFP, Sp.pCas9-dCas9, and BBa_J23107-MCP-SoxS(R93A/S101A) with miniTn7T method
    Depositor
    Jesse Zalatan
    Article
    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(
    Inserts
    J1-BBa_J23117-sfGFP
    Sp.pCas9-dCas9_BBa_J23107-MCP-SoxS(R93A, S101A)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    SoxS has R93A and S101A mutations
    Promoter
    J1-BBa_J23117 and Sp.pCas9, BBa_J23107
    Available since
    Oct. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171139-plasmid-map-sequence-id-344756

    Enlarge

  49. pORTMAGE311B

    Plasmid
    #120418
    Purpose
    Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR
    Depositor
    Csaba Pál
    Article
    Szili et al bioRxiv (2018)
    Inserts
    MutL E32K
    Lambda Beta
    XylS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E32K mutation conferring dominant mutator phenoty…
    Promoter
    Pm
    Available since
    Feb. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    120418-plasmid-map-sequence-id-236013

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  50. pBP-ORF-xylE

    Plasmid
    #112780
    Purpose
    Encodes catechol-2,3-dioxygenase, from Pseudomonas putida TOL pWW0.
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    xylE open reading frame
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    112780-plasmid-map-sequence-id-218368

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Showing: 501 - 550 of 651 results