-
Plasmid#125581PurposeFlp-dependent expression of PSD d1.2-GFP (a deletion mutant of PSD-95 fused to GFP) in mammalian cells.DepositorInsertPSDd1.2-GFP (Dlg4 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#2
Plasmid#107730PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #2 (targets Exon 1) (MAPRE3 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#1
Plasmid#107729PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3
Plasmid#107731PurposeKnock out of EB3 in human cells by CRISPR/Cas9DepositorInsertMAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-RIPK3 4KR
Plasmid#78825PurposeTo detect localization of RIPK3 4KR protein in live mammalian cellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsGFPExpressionMammalianMutationK55R, K89R, K363R, K501RPromoterCMVAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a W118S
Plasmid#40042DepositorInserthSlp4-a W118S (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationTryptophane 118 to SerinePromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a V21A
Plasmid#40041DepositorInserthSlp4-a V21A (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationValine 21 to AlaninePromoterCMV promoterAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-RATA
Plasmid#184270PurposeExpresses shRNA resistant GFP-RepoMan RATA MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTags3xHA and GFPExpressionMammalianMutationV383A, F395APromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-T394A
Plasmid#204994PurposeExpresses shRNA resistant GFP-RepoMan T394A MutantDepositorInsertcell devision cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationchanged Threonine 394 to AlaninPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-S893D
Plasmid#204995PurposeExpresses shRNA resistant GFP-RepoMan S893D MutantDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTagsGFPExpressionMammalianMutationchanged Serine 893 to Aspartic acidPromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 AURKA deltaNter
Plasmid#157753PurposeExpression of AURKA without Nter, fused to GFP in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
RCH1:GA2ox2-GFP
Plasmid#108567PurposeArabidopsis transformation, expresses GA2ox2 with RCH1 promoter to study expression pattern in rootsDepositorInsertGA2ox2 (GA2OX2 Mustard Weed)
UseTagsExpressionPlantMutationPromoterRCH1Available sinceJune 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHDT1:HDT1-GFP
Plasmid#108565PurposeArabidopsis transformation, expresses HDT1/GFP fusion with HDT1 promoter to study expression pattern in rootsDepositorInsertHDT1 (HDA3 Mustard Weed)
UseTagsGFPExpressionPlantMutationPromoterHDT1Available sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N2-RIPK3 V460P
Plasmid#78826PurposeTo detect localization of RIPK3 V460P protein in live mammalian cellsDepositorInsertRIPK3 (RIPK3 Human)
UseTagsGFPExpressionMammalianMutationV460PPromoterCMVAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a I18A
Plasmid#40043DepositorInserthSlp4-a I18A (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationIsoleucine 18 to AlaninePromoterCMV promoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a linker
Plasmid#40040DepositorInserthSlp4-a linker (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationlinker domain (144-353)PromotercmvAvailable sinceOct. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
PgyrA_gfp
Plasmid#212146PurposePlasmid expressing green fluorescent protein under constitutive promoter PgyrA control.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCMV-AcGFP
Plasmid#131008PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a CMV promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpCMV and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
p23-puro-EGFP-RPA194
Plasmid#179237Purposeoverexpression in human cellsDepositorInsertRPA194 (POLR1A Human)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pGFAP-AcGFP
Plasmid#133733PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a GFAP promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpGFAP and pTRE3G-BiAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-ArchT-GFP
Plasmid#29777PurposeAAV-mediated expression of ArchT-GFP for neuronal inhibitionDepositorInsertArchT-GFP
UseAAV; Adeno-associated virusTagsGFPExpressionMammalianMutationN/APromoterCAGAvailable sinceJuly 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS306-pHIS3-2xGFP-tURA3
Plasmid#116936PurposeYeast integrative vector for the expression of 2xGFPDepositorInsert2xGFP
UseTagsExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ArchT-GFP
Plasmid#63141PurposeAAV-mediated expression of ArchT-GFP under the Syn promoter. Using hGHpA signal.DepositorInsertArchT-GFP
UseAAVTagsGFPExpressionMammalianMutationPromoterhuman synapsin promoterAvailable sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-Pard3-EGFP-PIF6
Plasmid#154914PurposeFull length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).DepositorInsertpar-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
UseXenopus/avian/zebrafishTags1-100 amino acids of Arabidopsis PIF6 and EGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-ArchT-GFP
Plasmid#37807PurposeViral expression of ArchT-GFP for optogenetic inhibitionDepositorInsertArchT-GFP
UseAAV; Adeno-associated virusTagsGFPExpressionMammalianMutationPromoterCamKIIAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-gePSI-pCamk2a-AcGFP
Plasmid#133732PurposeExpresses both the gePSI-α-chain and the gePSI-β-chain under control of the inducible bi-directional TRE3G promoter. Expresses constitutively AcGFP under control of a Camk2a promoter.DepositorInsertsgePSI-β-chain
gePSI-α-chain
AcGFP1
UseAAVTagsmurine ornithine decarboxylase - degron (ODC36)ExpressionMammalianMutationPromoterpCamk2a and pTRE3G-BiAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 GFP RagC (CTD)
Plasmid#112768Purposeexpress GFP-RagC (C terminal domain only)DepositorInsertGFP-RagC (C terminal domain only) (RRAGC Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Abr R683A N795A
Plasmid#36417DepositorInsertactive BCR-related (ABR Human)
UseTagsEGFPExpressionMammalianMutationR683A N795APromoterAvailable sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
H2B-GFP-INCENP∆cen
Plasmid#184044PurposeExpresses H2B-GFP fused to INCENP∆cenDepositorInsertINCENP (INCENP Human)
UseTagsGFP and H2BExpressionMammalianMutationTruncated to 1-862PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
tet-pLKO.puro_shGFP
Plasmid#110421PurposeshGFP control, silence GFP gene, doxycycline inducible, puromycin selectionDepositorInsertGFP
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1/TO (RNA PolIII)Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-OPTN (2-119)
Plasmid#208867PurposeStably express GFP-tagged OPTN (2-119aa) for CID induced mitophagyDepositorInsertOPTN (OPTN Human)
UseTagsFKBP-GFPExpressionMammalianMutationaa 2-119 onlyPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWPI-IRES-eGFP-Mefv iso1
Plasmid#92104PurposeExpression in eukaryotic cells and generating stable cell linesDepositorInsertMefv isoform1 (Mefv Mouse)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP-LAMA-S98 (for p24)
Plasmid#130717PurposePlasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid proteinDepositorInsertEGFP-LAMA-S98
UseTagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE
Plasmid#198514PurposeExpresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.DepositorInsertPdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACTN3-pEGFP
Plasmid#52670PurposeOverexpression of GFP-tagged α-actinin-3DepositorInsertACTN3 (ACTN3 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
p1.2-GS-eGFP
Plasmid#162771PurposeFluorescent reporter for CHO expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 mitoAURKA
Plasmid#157754PurposeExpression of AURKA:GFP targeted to mitochondriaDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionMammalianMutationAURKA without the first 30 amino acids replaced b…PromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-a K>Q
Plasmid#40044DepositorInserthSlp4-a K>Q (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationLysine 410,412,416,564,566,571, 572 to GlutaminePromoterCMV promoterAvailable sinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp4-5-4a
Plasmid#40045DepositorInserthSlp4-5-4a (SYTL4 Human)
UseTagsEGFPExpressionMammalianMutationSlp4 linker substituted by Slp5 linker domain (s…PromoterCMV promoterAvailable sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Btsz2
Plasmid#40068DepositorInsertBtsz2 (btsz Fly)
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RepoMan-wt
Plasmid#184269PurposeExpresses shRNA resistant GFP-RepoManDepositorInsertcell division cycle associated 2 (CDCA2 Human)
UseTags3xHA and GFPExpressionMammalianMutationchanged nucleotides 723-747, 1488-1509, 1524-1548…PromoterCMVAvailable sinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT22UbGfpnE4PCh(#301)
Plasmid#184066Purposecyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenicDepositorInserteGFP-nls-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)TagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP N1 AURKA 1-30
Plasmid#157756PurposeExpression of AURKA Nter (aa1-30):GFP in mammalian cellsDepositorInsertAURKA (AURKA Human)
UseTagsGFPExpressionMammalianMutationThe first 30 amino acids of AURKA were clonedPromoterCMVAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-GFP/BFP(LaG2) PAGER(FL)
Plasmid#230022PurposeExpresses anti-GFP/BFP PAGER(FL) in mammalian cellsDepositorInsertpAAV-MT3-LaG2-TEVcs-ALFA-hM4DxcpGFP
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-EGFP-mir30(Scn9a-scrambled)
Plasmid#79671PurposeScrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)DepositorInsertEGFP
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-eGFP-P2A-CRY2-VPR
Plasmid#192610PurposeLentiviral vector for constitutive expression of eGFP-P2A-CRY2-VPR.DepositorInserteGFP-P2A-CRY2PHR-VPR
UseLentiviral and Synthetic BiologyTagseGFPExpressionMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE
Plasmid#32702PurposeConditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.DepositorInserteGFP
UseCre/Lox and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)
Plasmid#174639PurposeOptogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)DepositorInsertSSPB(micro)-GFP-ppKinVIb(861-1321)
UseTagsSSPB(micro)-GFPExpressionMammalianMutationSSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…PromoterChicken beta-actinAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
4xPylT(AGGA)Ev2 PylS mCherry-P2A-eGFP(AGGA)
Plasmid#174529PurposeDual-fluorescence reporter for quantifying PylT(AGGA)Ev2 decoding efficiencyDepositorInsertsPylS
PylT
UseTagsFlagExpressionMammalianMutationPromoterU6Available sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-I-LSS-SGFP2
Plasmid#112945PurposeProduces a fusion between LSS-GFP2 and mScarlet-I. It can be used as a positive control for FRETDepositorInsertLSS-GFP2 mScarlet-I Fusion
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only