Addgene

We narrowed to 505 results for: otc

Showing: 401 - 440 of 505 results

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CEBPalpha NGFR

Plasmid

#44627

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

CEBPalpha (Cebpa Mouse)

Use

Retroviral

Tags

IRES and truncated NGFR

Expression

Mammalian

Mutation

Promoter

Available since

April 30, 2013

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_hGH

Plasmid

#182410

Purpose

Bacterial expression of human growth hormone

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

hGH (GH1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p6874 pHAGE-N-V5-MAML1-ADDAA

Plasmid

#37050

Depositor

Peter Howley

Article

Tan et al Proc Natl Acad Sci U S A. 2012 Apr 30.

Insert

MAML1-ADDAA (MAML1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMVt

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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Dll1ICD-HPC4 pBacPAK8-3

Plasmid

#17333

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Delta-like 1 (Dll1 Mouse)

Use

Tags

HPC4

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-DARPinOFF7

Plasmid

#182397

Purpose

Bacterial expression of Vesicle Nucleating peptide-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DARPinOFF7

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Jag2ICD-HA pBacPAK8-3

Plasmid

#17332

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Jagged2 (Jag2 Rat)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
Jag1ICD-HA pBacPAK8-3

Plasmid

#17331

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Jagged1 (Jag1 Rat)

Use

Tags

HA

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
Dll3ICD-HPC4 pBacPAK8-3

Plasmid

#17334

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Delta-like 3 (Dll3 Mouse)

Use

Tags

HPC4

Expression

Insect

Mutation

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
Bambi NGFR

Plasmid

#44628

Depositor

Ellen Rothenberg

Article

Del Real et al Development. 2013 Mar;140(6):1207-19. do

Insert

Bambi (Bambi Mouse)

Use

Retroviral

Tags

IRES and truncated NGFR

Expression

Mammalian

Mutation

Promoter

Available since

April 17, 2013

Availability

Academic Institutions and Nonprofits only
Neurl1-FLAG/Jag1-HA pIRES

Plasmid

#17337

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
Neurl1G2A-Flag pcDNA3.1

Plasmid

#17320

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

RNAi

Tags

FLAG

Expression

Mammalian

Mutation

Gly 2 changed to Ala

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_FGF21

Plasmid

#182406

Purpose

Bacterial expression of fgf21

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

fgf21 (FGF21 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1_VNp-LZ_CydAB-mNeongreen

Plasmid

#182393

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ
CydAB-mNeongreen

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pNCMV HPV8 E6 del132-136

Plasmid

#87658

Purpose

Flag/HA tagged HPV8 E6 del132-136

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Use

Tags

Flag and HA

Expression

Mammalian

Mutation

Deletion of aa132-136

Promoter

CMV

Available since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Neurl1Rm-Flag pBacPAK8-3

Plasmid

#17326

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

Neuralized-like (Neurl1a Mouse)

Use

Tags

FLAG

Expression

Insect

Mutation

Cys 521 changed to Ser Cys 524 changed to Ser

Promoter

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_mNeongreen-Etanercept

Plasmid

#182420

Purpose

Bacterial expression of mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-Etanercept (TNFRSF1B Human)

Use

Tags

mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR-Cre stuffer v4

Plasmid

#158033

Purpose

lenti-viral construct with Cas9, Cre recombinase and U6 driven sgRNA casette with modified tracr RNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI)

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pmROSA26-attP50-Neo-mKate2-3xNLS-attP50

Plasmid

#183609

Purpose

Targeting vector for mouse ROSA26 locus to create landing pad cell line. Introduces attP50 sites flanking a Neo gene driven by endogenous locus (confers G418 resistance) and a CAG-mKate2-3xNLS.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Inserts

Neo
mKate2-3xNLS

Use

Mouse Targeting

Tags

3xNLS

Expression

Mutation

Promoter

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-Cre sgAmotl2 v3

Plasmid

#158602

Purpose

lenti-viral construct with Cas9, Cre recombinbase and U6 driven sgRNA against mouse Amotl2

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Amotl2 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLKO-CreERT2 stuffer v3

Plasmid

#158046

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLKO-Cre stuffer v4

Plasmid

#158032

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pRSFDuet-1_VNp15-StefinA

Plasmid

#182405

Purpose

Bacterial expression of Vesicle Nucleating peptide15-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-stefin (CSTA Human)

Use

Tags

Vesicle Nucleating peptide 15 (VNp15)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

Plasmid

#182415

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-DARPinOFF7

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1_VNp6-StefinA

Plasmid

#182404

Purpose

Bacterial expression of Vesicle Nucleating peptide6-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-stefin (CSTA Human)

Use

Tags

Vesicle Nucleating peptide 6 (VNp6)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPyPGK-HA-EGFP-Myc-TM-IRES-Pac

Plasmid

#183606

Purpose

Mammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

EGFP-TM

Use

Tags

HA, Human PDGFRB transmembrane domain, Mouse IgGK…

Expression

Mammalian

Mutation

Promoter

Mouse Pgk1

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1_VNp-StefinA

Plasmid

#182403

Purpose

Bacterial expression of Vesicle Nucleating peptide-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-stefin (CSTA Human)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-Cre sgAdam10 v3

Plasmid

#158034

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Adam10

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
2xHA MED10 pBacPAK8

Plasmid

#17321

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2007 Dec 12. ():.

Insert

MED10 (Med10 Mouse)

Use

Tags

2xHA

Expression

Insect

Mutation

Promoter

Available since

March 11, 2008

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-mNeongreen-Uricase

Plasmid

#182417

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-uricase

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1_mNeongreen-StefinA

Plasmid

#182418

Purpose

Bacterial expression of mNeongreen-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-stefin (CSTA Human)

Use

Tags

mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
attB53-Pac-tetO-mCherry-attB53

Plasmid

#183612

Purpose

RMCE vector to shuttle puromycin resistance and tetO-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

tetO-mCherry

Use

Rmce

Tags

Expression

Mutation

Promoter

tetO

Available since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO-Cre sgUroD v4

Plasmid

#158036

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

UroD sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLKO-Cre sgItgb5 v3

Plasmid

#158038

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Itgb5 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLKO-Cre sgRipk4 v3

Plasmid

#158035

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Ripk4 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
jam2a-pCRII

Plasmid

#68804

Purpose

For in situ antisense probe, NotI w/ Sp6

Depositor

David Traver

Article

Kobayashi et al Nature. 2014 Aug 21;512(7514):319-23. do

Insert

jam2a (jam2a Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only
pRSFDuet-1_VNp-DNAseI

Plasmid

#182409

Purpose

Bacterial expression of Vesicle Nucleating peptide-DNaseI fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-DNase I (DNASE1 Bovine)

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pB-TAG-NICD

Plasmid

#130934

Purpose

PiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells

Depositor

James Thomson

Article

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

NICD (NOTCH1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TetO

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF3135

Plasmid

#144611

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NOTCH2 (NOTCH2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-1_VNp-mNeongreen-Etanercept

Plasmid

#182421

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-Etanercept (TNFRSF1B Human)

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
DBD (N208AH212R) Foxo1-WT pDNR-CMV

Plasmid

#17555

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

Insert

Foxo1-WT-DBD (Foxo1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

DNA binding-deficientChanged N208A H212R

Promoter

CMV

Available since

Oct. 20, 2008

Availability

Academic Institutions and Nonprofits only

Showing: 401 - 440 of 505 results

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