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Showing: 301 - 330 of 6364 results

  15. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

    Plasmid
    #192552
    Purpose
    Expresses nuclear localized tdTomato under CAG promoter
    Depositor
    Guoping Feng
    Has Service
    AAV PHP.eB and AAV9
    Insert
    tdTomato
    Use
    AAV
    Tags
    NLS
    Expression
    Mutation
    deleted E273 and G274 compared to FPbase ID: PGG5S
    Promoter
    CAG
    Available since
    Nov. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    192552-plasmid-map-sequence-id-403193

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  17. Recruited polymerase expression - pCMV-T7-EcKlenow-CC(N5) (LM2696)

    Plasmid
    #208969
    Purpose
    Recruited EcKlenow DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    EcKlenow-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208969-plasmid-map-sequence-id-415816

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  18. pCIneo-hCHD7-Kozak ATG 3' HA-bGH polyA

    Plasmid
    #89460
    Purpose
    constitutive expression of human CHD7 in mammalian cells
    Depositor
    Peter Scacheri
    Insert
    CHD7 (CHD7 Human)
    Use
    Tags
    hinge + HA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    89460-plasmid-map-sequence-id-176096

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  21. RNA polymerase beta prime subunit FLAG

    Plasmid
    #102337
    Purpose
    Targeting plasmids for replacing the S. elongatus PCC 7942 genomic copy of Synpcc7942_1524 (Beta prime subunit of RNA polymerase) with a C-terminal FLAG tagged variant.
    Depositor
    Erin O'Shea
    Article
    Piechura et al Elife. 2017 Dec 14;6. pii: 32032. doi: 1
    Insert
    RNAP Beta Prime Subunit with C-terminal FLAG TAG
    Use
    Tags
    3x FLAG with 3X GSGS linker
    Expression
    Mutation
    Promoter
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    102337-plasmid-map-sequence-id-197499

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  23. IX301: pMVP (L3-L2) myc epitope tag + polyA

    Plasmid
    #121753
    Purpose
    pMVP L3-L2 entry plasmid, contains myc epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    myc epitope tag + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121753-plasmid-map-sequence-id-237192

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  24. IX601: pMVP (L3-L2) FLAG epitope tag + polyA

    Plasmid
    #121751
    Purpose
    pMVP L3-L2 entry plasmid, contains FLAG epitope tag + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    FLAG epitope tag + polyA
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121751-plasmid-map-sequence-id-237190

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  25. pAAV-EF1-fDIO-mNeon-WPRE-PolyA

    Plasmid
    #121546
    Purpose
    Super-bright fluorescence protein for tracking and visualization
    Depositor
    Matthias Prigge
    Insert
    cre-dependent mNeon
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    121546-plasmid-map-sequence-id-308125

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  26. C513-E07: attB5r-<p10p-polyhedrinp>-attB1r

    Plasmid
    #162949
    Purpose
    Gateway attB5r/attB1r entry clone containing divergent AcMNPV polyhedrin and p10 promoters, for use in construction of polycistronic baculovirus expression vectors
    Depositor
    Dominic Esposito
    Article
    Wall et al Methods Mol Biol. 2014;1116:193-208. doi
    Insert
    dual baculovirus promoter cassette (AcMNPV p10 and polyhedrin)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    162949-plasmid-map-sequence-id-330549

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  28. pGL3E-hGPR56 e1m promoter perisylvian polymicrogyria

    Plasmid
    #52299
    Purpose
    Reporter for mutated human GPR56 exon 1m promoter activity. It contains a 15-bp deletion in a conserved noncoding element. The mutated human GPR56 e1m promoter was inserted into pGL3E.
    Depositor
    Christopher A Walsh
    Article
    Bae et al Science. 2014 Feb 14;343(6172):764-8. do
    Insert
    ADGRG1 adhesion G protein-coupled receptor G1 (ADGRG1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    15-bp deletion in the promoter
    Promoter
    Human GPR56 e1m promoter
    Available since
    April 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    52299-plasmid-map-sequence-id-80926

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Showing: 301 - 330 of 6364 results