Addgene

We narrowed to 1,445 results for: Mbp

Showing: 301 - 330 of 1445 results

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GST-TEVcs-FIP200-MBP

Plasmid

#171410

Purpose

For the purifcation of human ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

FIP200 (RB1CC1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDB.His.MBP.3C CARD8 UPA-CARD WT

Plasmid

#164021

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CARD8 (CARD8 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-RGG-SmBit-RGG

Plasmid

#176644

Purpose

SmBit fused to LAF-1 RGG domain with N-terminal MBP tag

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

MBP-RGG-SmBit-RGG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1048A_H1053A

Plasmid

#83484

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 2 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R1048A_H1053A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R1048A, H1053A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-CB2-HF

Plasmid

#121682

Purpose

Bacterial expression of cannabinoid receptor CB2 fusion protein

Depositor

Reinhard Grisshammer, Richard Henderson

Article

Calandra et al Biotechnology Letters

Insert

peripheral cannabinoid receptor (CNR2 Human)

Use

Tags

His6 Flag tag

Expression

Bacterial

Mutation

Promoter

lac

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Ppr_Cas13a_WT

Plasmid

#91872

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

PprCas13a (PprC2c2) WT

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lne_Cas13a_WT

Plasmid

#91866

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LneCas13a (LneC2c2) WT

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1079A_K1080A

Plasmid

#91861

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) R0179A/K1080A

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1080A

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_E299A

Plasmid

#91855

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) E299A

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

E299A

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R472A_H477A

Plasmid

#83483

Purpose

Bacterial expression of L. buccalis C2c2 CRISPR effector (HEPN nuclease 1 inactive mutant).

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_R472A_H477A

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

R472A, H477A

Promoter

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pSL0373 (His-MBP-TniQ)

Plasmid

#130638

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite tag for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Rca_Cas13a_WT

Plasmid

#91870

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

RcaCas13a (RcaC2c2) WT

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET_His6-MBP-TEV-Cas2

Plasmid

#186275

Purpose

Expresses Thiomicrospira Cas2 in E. coli

Depositor

Jennifer Doudna

Article

Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

Insert

Thiomicropira Cas2

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pET-MBP-CfaN-His6

Plasmid

#126017

Purpose

Bacterial expression of MBP-CfaN fusion protein (with C-terminal His5)

Depositor

Thomas Muir

Insert

MBP-CfaN-His5

Use

Tags

5xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Cam_Cas13a_WT

Plasmid

#91869

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

CamCas13a (CamC2c2) WT

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Ere_Cas13a_WT

Plasmid

#91868

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

EreCas13a (EreC2c2) WT

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K1087A

Plasmid

#91864

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1087A

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1087A

Promoter

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pDB-His-MBP-SARM1

Plasmid

#195762

Purpose

Purification of SARM1 from E.coli

Depositor

Hao Wu

Insert

SARM1 (SARM1 Human)

Use

Tags

HIS-MBP-TEV

Expression

Bacterial

Mutation

truncation of first 19 residues

Promoter

T7

Available since

Feb. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a-MBP-(CBX1)3-eReader

Plasmid

#135090

Purpose

Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered x3

Use

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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MBP-GTSE1(long)-His6

Plasmid

#69110

Purpose

For bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.

Depositor

Stephen Royle

Article

Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

Insert

G2- and S-phase Expressed 1 (GTSE1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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MBP-TSF-TEVcs-ULK1K46I

Plasmid

#171417

Purpose

For the purifcation of human ULK1 kinase dead complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ULK1 (ULK1 Synthetic)

Use

Tags

Expression

Mammalian

Mutation

kinase dead

Promoter

Available since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_R1072A

Plasmid

#91859

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) R1072A

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

R1072A

Promoter

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K1082A

Plasmid

#91863

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1082A

Use

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1082A

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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MBP-RGG-LgBit-RGG

Plasmid

#176643

Purpose

LgBit fused to LAF-1 RGG domain with N-terminal MBP tag

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

MBP-RGG-LgBit-RGG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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M47 MBP-TEV-Chibby

Plasmid

#17195

Depositor

Randall Moon

Article

Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

Insert

Chibby (CBY1 Human)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pDB.His.MBP.3C NLRP1 UPA-CARD WT

Plasmid

#164003

Purpose

Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged NLRP1 UPA-CARD

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

NLRP1 (NLRP1 Human)

Use

Tags

His6-MBP-3C

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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His-MBP-NRBF2 (1-159)

Plasmid

#99331

Purpose

Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

Depositor

James Hurley

Article

Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

Insert

Nuclear receptor-binding factor 2 (NRBF2 Human)

Use

Tags

6xHis-MBP-TEV

Expression

Bacterial

Mutation

AA1-159 are present

Promoter

T7

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-AtTIR1-Myc-MBP-HA

Plasmid

#222709

Purpose

Expression AtTIR1-Myc and MBP-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtTIR1 (TIR1 Mustard Weed)

Use

Tags

myc

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-Myc-MBP-AtIAA6-HA

Plasmid

#222710

Purpose

Expression MBP-AtIAA6-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Insert

AtIAA6 (IAA6 Mustard Weed)

Use

Tags

HA and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Plasmid

#222990

Purpose

Bacterial expression of iGeoCas9(C1) construct

Depositor

Jennifer Doudna

Article

Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

Insert

His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

Use

CRISPR

Tags

CL7, His, and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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