pRG/III-hs-MBP-CB2-HF
(Plasmid #121682)

• Purpose: Bacterial expression of cannabinoid receptor CB2 fusion protein
• Depositing Labs: Reinhard Grisshammer, Richard Henderson
• Publication: Calandra et al Biotechnology Letters

Backbone

• Vector backbone: pRG/III-hs-MBP
• Backbone size w/o insert (bp): 6991
• Total vector size (bp): 8141
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: peripheral cannabinoid receptor
• Alt name: CB2
• Species: H. sapiens (human)
• Insert Size (bp): 1092
• Entrez Gene: CNR2 (a.k.a. CB-2, CB2, CX5)
• Promoter: lac
• Tag / Fusion Protein:
  • His6 Flag tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: MalE2
• 3′ sequencing primer: M13 forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Internal plasmid code # 611

This plasmid is in fact published: Calandra 1997, see uploaded paper. The citation however does not show in PubMed.